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Plant Extracellular Vesicles Contain Diverse Small RNA Species and Are Enriched in 10 to 17 Nucleotide “Tiny” RNAs

View ORCID ProfilePatricia Baldrich, View ORCID ProfileBrian D. Rutter, View ORCID ProfileHana Zandkarimi, View ORCID ProfileRam Podicheti, View ORCID ProfileBlake C. Meyers, View ORCID ProfileRoger W. Innes
doi: https://doi.org/10.1101/472928
Patricia Baldrich
aDonald Danforth Plant Science Center, St. Louis, Missouri 63132
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Brian D. Rutter
bDepartment of Biology, Indiana University, Bloomington, Indiana 47405
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Hana Zandkarimi
bDepartment of Biology, Indiana University, Bloomington, Indiana 47405
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Ram Podicheti
bDepartment of Biology, Indiana University, Bloomington, Indiana 47405
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Blake C. Meyers
aDonald Danforth Plant Science Center, St. Louis, Missouri 63132
cUniversity of Missouri-Columbia, Division of Plant Sciences, Columbia, Missouri 65211
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  • For correspondence: bmeyers@danforthcenter.org rinnes@indiana.edu
Roger W. Innes
bDepartment of Biology, Indiana University, Bloomington, Indiana 47405
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  • For correspondence: bmeyers@danforthcenter.org rinnes@indiana.edu
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ABSTRACT

Small RNAs (sRNAs) that are 21 to 24 nucleotides (nt) in length are found in most eukaryotic organisms and regulate numerous biological functions, including transposon silencing, development, reproduction, and stress responses, typically via control of the stability and/or translation of target mRNAs. Major classes of sRNAs in plants include microRNAs (miRNAs) and small interfering RNAs (siRNAs); sRNAs are known to travel as a silencing signal from cell to cell, root to shoot, and even between host and pathogen. In mammals, sRNAs are transported inside extracellular vesicles (EVs), which are mobile lipid compartments that participate in intercellular communication. In addition to sRNAs, EVs carry proteins, lipids, metabolites, and potentially other types of nucleic acids. Here we report that plant EVs also contain diverse species of sRNA. We found that specific miRNAs and siRNAs are preferentially loaded into plant EVs. We also report a previously overlooked class of “tiny RNAs” (10 to 17 nt) that are highly enriched in EVs. This new RNA category of unknown function has a broad and very diverse genome origin and might correspond to degradation products.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted November 17, 2018.
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Plant Extracellular Vesicles Contain Diverse Small RNA Species and Are Enriched in 10 to 17 Nucleotide “Tiny” RNAs
Patricia Baldrich, Brian D. Rutter, Hana Zandkarimi, Ram Podicheti, Blake C. Meyers, Roger W. Innes
bioRxiv 472928; doi: https://doi.org/10.1101/472928
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Plant Extracellular Vesicles Contain Diverse Small RNA Species and Are Enriched in 10 to 17 Nucleotide “Tiny” RNAs
Patricia Baldrich, Brian D. Rutter, Hana Zandkarimi, Ram Podicheti, Blake C. Meyers, Roger W. Innes
bioRxiv 472928; doi: https://doi.org/10.1101/472928

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