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Excess light priming in Arabidopsis thaliana genotypes with altered DNA methylomes

View ORCID ProfileDiep R. Ganguly, View ORCID ProfileBethany A. B. Stone, View ORCID ProfileAndrew F. Bowerman, View ORCID ProfileSteven R. Eichten, View ORCID ProfileBarry J. Pogson
doi: https://doi.org/10.1101/475798
Diep R. Ganguly
Australian Research Council Centre of Excellence in Plant Energy Biology, Research School of Biology, The Australian National University Canberra, Acton, ACT, 2601, Australia
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Bethany A. B. Stone
Australian Research Council Centre of Excellence in Plant Energy Biology, Research School of Biology, The Australian National University Canberra, Acton, ACT, 2601, Australia
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Andrew F. Bowerman
Australian Research Council Centre of Excellence in Plant Energy Biology, Research School of Biology, The Australian National University Canberra, Acton, ACT, 2601, Australia
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Steven R. Eichten
Australian Research Council Centre of Excellence in Plant Energy Biology, Research School of Biology, The Australian National University Canberra, Acton, ACT, 2601, Australia
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Barry J. Pogson
Australian Research Council Centre of Excellence in Plant Energy Biology, Research School of Biology, The Australian National University Canberra, Acton, ACT, 2601, Australia
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  • For correspondence: barry.pogson@anu.edu.au
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Abstract

Plants must continuously react to the ever-fluctuating nature of their environment. Repeated exposure to stressful conditions can lead to priming, whereby prior encounters heighten a plant’s ability to respond to future events. A clear example of priming is provided by the model plant Arabidopsis thaliana (Arabidopsis), in which photosynthetic and photoprotective responses are enhanced following recurring light stress. While there are various post-translational mechanisms underpinning photoprotection, an unresolved question is the relative importance of transcriptional changes towards stress priming and, consequently, the potential contribution from DNA methylation – a heritable chemical modification of DNA capable of influencing gene expression. Here, we systematically investigate the potential molecular underpinnings of physiological priming against recurring excess-light (EL), specifically DNA methylation and transcriptional regulation: the latter having not been examined with respect to EL priming. The capacity for physiological priming of photosynthetic and photoprotective parameters following a recurring EL treatment was not impaired in Arabidopsis mutants with perturbed establishment, maintenance, or removal of DNA methylation. Importantly, no differences in development or basal photoprotective capacity were identified in the mutants that may confound the above result. Little evidence for a causal transcriptional component of physiological priming was identified; in fact, most alterations in primed plants presented as a transcriptional ‘dampening’ in response to an additional EL exposure, likely a consequence of physiological priming. However, a set of transcripts uniquely regulated in primed plants provide preliminary evidence for a novel transcriptional component of recurring EL priming, independent of physiological changes. Thus, we propose that physiological priming of recurring EL in Arabidopsis occurs independently of DNA methylation; and that the majority of the associated transcriptional alterations are a consequence, not cause, of this physiological priming.

One sentence summary Photoprotection and priming against recurring excess light is functional despite impaired maintenance of the DNA methylome.

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  • https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE121150

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted July 05, 2019.
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Excess light priming in Arabidopsis thaliana genotypes with altered DNA methylomes
Diep R. Ganguly, Bethany A. B. Stone, Andrew F. Bowerman, Steven R. Eichten, Barry J. Pogson
bioRxiv 475798; doi: https://doi.org/10.1101/475798
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Excess light priming in Arabidopsis thaliana genotypes with altered DNA methylomes
Diep R. Ganguly, Bethany A. B. Stone, Andrew F. Bowerman, Steven R. Eichten, Barry J. Pogson
bioRxiv 475798; doi: https://doi.org/10.1101/475798

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