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Early detection of retinal alteration by visible and near-infrared optical co-herence tomography (vnOCT) in a dexamethasone-induced ocular hypertension mouse model

View ORCID ProfileWeiye Song, Sipei Fu, Shangshang Song, Sui Zhang, Lei Zhang, Steven Ness, Manishi Desai, Ji Yi
doi: https://doi.org/10.1101/478719
Weiye Song
1Department of Medicine, Boston University School of Medicine, Boston Medical Center, Boston MA, 02118
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Sipei Fu
2Department of Biology, Boston University, Boston MA, 02215
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Shangshang Song
3Department of Health Science, Boston University Sargent School of Rehabilitation, Boston MA, 02215
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Sui Zhang
4Danna-Farber Cancer Institute, Boston MA, 02215, USA
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Lei Zhang
1Department of Medicine, Boston University School of Medicine, Boston Medical Center, Boston MA, 02118
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Steven Ness
5Department of Ophthalmology, Boston Medical Center, Boston MA, 02118
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Manishi Desai
5Department of Ophthalmology, Boston Medical Center, Boston MA, 02118
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Ji Yi
1Department of Medicine, Boston University School of Medicine, Boston Medical Center, Boston MA, 02118
6Department of Biomedical Engineering, Boston University, Boston MA, 02215
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  • For correspondence: jiyi@bu.edu
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ABSTRACT

Purpose To apply a novel visible and near-infrared optical coherence tomography (vnOCT) in the dexa-methasone-induced ocular hypertension mouse model, and test the capability of four optical markers, peri-papillary retinal nerve fiber layer (RNFL) thickness, total retinal blood flow, VN ratio and hemoglobin oxygen saturation (sO2), in detecting retinal ganglion cell (RGC) damage in association with ocular hyper-tension.

Methods Twelve mice (C57BL/6J) were separated into a control (n=6) and a dexamethasone group (n=6) receiving twice daily saline or dexamethasone eye drops, respectively, for 7 weeks. Intraocular pressure (IOP) measurements were taken at baseline and weekly. Optical measurements by vnOCT were longitudinally taken at baseline, 4 weeks and 7 weeks. Following week 7, ex vivo RGC counting was performed by immunostaining.

Results The dexamethasone group showed a measurable rise in IOP by week 2. Despite the IOP differences between the dexamethasone and control groups, there was not a statistical difference in RNFL thickness or total blood flow over 7 weeks. The dexamethasone group did show an increase in retinal arteriovenous sO2 difference (A-V sO2) that was significant at week 4 and 7. The RNFL VN ratio showed a significant decrease at week 4 and 7 in dexamethasone group associated with a decreased RGC count.

Conclusions RNFL VN ratio and A-V sO2 are capable of detecting early retinal alterations in the dexamethasone-induced ocular hypertension mouse model. Data analysis suggests VN ratio and A-V sO2 are corralated with RGC loss secondary to ocular hypertension, while being independent of IOP.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted November 29, 2018.
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Early detection of retinal alteration by visible and near-infrared optical co-herence tomography (vnOCT) in a dexamethasone-induced ocular hypertension mouse model
Weiye Song, Sipei Fu, Shangshang Song, Sui Zhang, Lei Zhang, Steven Ness, Manishi Desai, Ji Yi
bioRxiv 478719; doi: https://doi.org/10.1101/478719
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Early detection of retinal alteration by visible and near-infrared optical co-herence tomography (vnOCT) in a dexamethasone-induced ocular hypertension mouse model
Weiye Song, Sipei Fu, Shangshang Song, Sui Zhang, Lei Zhang, Steven Ness, Manishi Desai, Ji Yi
bioRxiv 478719; doi: https://doi.org/10.1101/478719

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