Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Acute and chronic stage adaptations of vascular architecture and cerebral blood flow in a mouse model of TBI

Joe Steinman, Lindsay S. Cahill, Margaret M. Koletar, Bojana Stefanovic, John G. Sled
doi: https://doi.org/10.1101/479626
Joe Steinman
1Mouse Imaging Centre, The Hospital for Sick Children, Toronto, Ontario, Canada
2Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: joe.steinman@mail.utoronto.ca
Lindsay S. Cahill
1Mouse Imaging Centre, The Hospital for Sick Children, Toronto, Ontario, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Margaret M. Koletar
3Sunnybrook Research Institute, Toronto, Ontario, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Bojana Stefanovic
2Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada
3Sunnybrook Research Institute, Toronto, Ontario, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
John G. Sled
1Mouse Imaging Centre, The Hospital for Sick Children, Toronto, Ontario, Canada
2Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

The 3D organization of cerebral blood vessels determines the overall capacity of the cerebral circulation to meet the metabolic requirements of the brain. This study used Arterial Spin Labeling (ASL) MRI with a hypercapnic challenge and ex vivo Serial Two-Photon Tomography (STPT) to examine the relationship between blood flow and 3D microvascular structure following traumatic brain injury (TBI) in a mouse. Mice were exposed to a controlled cortical impact TBI and allowed to recover for either 1 day or 4 weeks. At each time point, ASL MRI was performed to quantify cerebral perfusion and the brain vasculature was imaged in 3D with STPT. Registration of ASL to STPT enabled flow changes to be related to the underlying microvascular structure in each ASL voxel. Hypoperfusion under rest and hypercapnia was observed both 1 day and 4 weeks post-TBI. Vessel density and vascular volume were reduced 1 day post-TBI, recovering by 4 weeks; however, the reorganized vasculature at the latter time point possessed an abnormal radial pattern. Our findings demonstrate functionally significant long-term changes in the vascular architecture following injury and illustrate why metrics beyond traditional measures of vessel density are required to understand the impact of vascular structure on function.

Footnotes

  • This work was supported by the Canadian Institutes of Health Research Grant FRN – 152961.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Back to top
PreviousNext
Posted November 27, 2018.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Acute and chronic stage adaptations of vascular architecture and cerebral blood flow in a mouse model of TBI
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Acute and chronic stage adaptations of vascular architecture and cerebral blood flow in a mouse model of TBI
Joe Steinman, Lindsay S. Cahill, Margaret M. Koletar, Bojana Stefanovic, John G. Sled
bioRxiv 479626; doi: https://doi.org/10.1101/479626
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
Acute and chronic stage adaptations of vascular architecture and cerebral blood flow in a mouse model of TBI
Joe Steinman, Lindsay S. Cahill, Margaret M. Koletar, Bojana Stefanovic, John G. Sled
bioRxiv 479626; doi: https://doi.org/10.1101/479626

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Neuroscience
Subject Areas
All Articles
  • Animal Behavior and Cognition (4237)
  • Biochemistry (9147)
  • Bioengineering (6786)
  • Bioinformatics (24020)
  • Biophysics (12137)
  • Cancer Biology (9544)
  • Cell Biology (13795)
  • Clinical Trials (138)
  • Developmental Biology (7642)
  • Ecology (11715)
  • Epidemiology (2066)
  • Evolutionary Biology (15518)
  • Genetics (10650)
  • Genomics (14332)
  • Immunology (9492)
  • Microbiology (22857)
  • Molecular Biology (9103)
  • Neuroscience (49029)
  • Paleontology (355)
  • Pathology (1484)
  • Pharmacology and Toxicology (2572)
  • Physiology (3848)
  • Plant Biology (8337)
  • Scientific Communication and Education (1472)
  • Synthetic Biology (2296)
  • Systems Biology (6196)
  • Zoology (1302)