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Human sperm chromatin forms spatially restricted nucleosome domains consistent with programmed nucleosome positioning

Mei-Zi Zhang, Xiao-Min Cao, Feng-Qin Xu, Xiao-Wei Liang, Long-Long Fu, Fang-Zhen Sun, Xiu-Ying Huang, View ORCID ProfileWei-Hong Huang
doi: https://doi.org/10.1101/481028
Mei-Zi Zhang
1Reproductive Medicine Center, Tianjin First central hospital, Tianjin, 300192, China
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Xiao-Min Cao
1Reproductive Medicine Center, Tianjin First central hospital, Tianjin, 300192, China
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Feng-Qin Xu
1Reproductive Medicine Center, Tianjin First central hospital, Tianjin, 300192, China
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Xiao-Wei Liang
2Bejing Human Sperm Bank and National Research Institute for Family Planning, Beijing, 100101, China
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Long-Long Fu
2Bejing Human Sperm Bank and National Research Institute for Family Planning, Beijing, 100101, China
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Fang-Zhen Sun
3State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
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Xiu-Ying Huang
3State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
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Wei-Hong Huang
3State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
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  • ORCID record for Wei-Hong Huang
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Abstract

In human sperm, a fraction of its chromatin retains nucleosomes that are positioned on specific sequences containing genes and regulatory units essential for embryonic development. This nucleosome positioning (NP) feature provides an inherited epigenetic mark for sperm. However, it is not known whether there is a structural constraint for these nucleosomes and, if so, how they are localized in a three-dimensional (3D) context of the sperm nucleus. In this study, we examine the 3D organization of sperm chromatin and specifically determine its 3D localization of nucleosomes using structured illumination microscopy. A fraction of the sperm chromatin form nucleosome domains (NDs), visible as microscopic puncta ranging from 40 μm to 700 μm in diameter, and these NDs are precisely localized in the postacrosome region (PAR), outside the sperm’s core chromatin. Further, NDs exist mainly in sperm from fertile men in a pilot survey with a small sample size. Together, this study uncovers a new spatially restricted sub-nuclear structure containing NDs that are consistent with NPs of the sperm, which might represent a novel mark for healthy sperm in human.

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Posted December 29, 2018.
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Human sperm chromatin forms spatially restricted nucleosome domains consistent with programmed nucleosome positioning
Mei-Zi Zhang, Xiao-Min Cao, Feng-Qin Xu, Xiao-Wei Liang, Long-Long Fu, Fang-Zhen Sun, Xiu-Ying Huang, Wei-Hong Huang
bioRxiv 481028; doi: https://doi.org/10.1101/481028
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Human sperm chromatin forms spatially restricted nucleosome domains consistent with programmed nucleosome positioning
Mei-Zi Zhang, Xiao-Min Cao, Feng-Qin Xu, Xiao-Wei Liang, Long-Long Fu, Fang-Zhen Sun, Xiu-Ying Huang, Wei-Hong Huang
bioRxiv 481028; doi: https://doi.org/10.1101/481028

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