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Modular, part-based control of gene expression response time using protein degradation tags

View ORCID ProfileEthan M Jones, View ORCID ProfileCallan E Monette, View ORCID ProfileJohn P Marken, View ORCID ProfileSejal Dhawan, View ORCID ProfileTheresa Gibney, View ORCID ProfileChristine Li, View ORCID ProfileWukun Liu, View ORCID ProfileAlyssa Luz-Ricca, View ORCID ProfileXida Ren, View ORCID ProfileXingyu Zheng, View ORCID ProfileMargaret Saha
doi: https://doi.org/10.1101/482331
Ethan M Jones
Department of Biology, Integrated Science Center, The College of William and Mary, 540 Landrum Drive, Williamsburg, VA 23185, United States
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Callan E Monette
Department of Biology, Integrated Science Center, The College of William and Mary, 540 Landrum Drive, Williamsburg, VA 23185, United States
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John P Marken
Division of Biology and Bioengineering, California Institute of Technology, Pasadena, CA 91125, United States
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Sejal Dhawan
Department of Biology, Integrated Science Center, The College of William and Mary, 540 Landrum Drive, Williamsburg, VA 23185, United States
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Theresa Gibney
Department of Biology, Integrated Science Center, The College of William and Mary, 540 Landrum Drive, Williamsburg, VA 23185, United States
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Christine Li
Department of Biology, Integrated Science Center, The College of William and Mary, 540 Landrum Drive, Williamsburg, VA 23185, United States
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Wukun Liu
Department of Biology, Integrated Science Center, The College of William and Mary, 540 Landrum Drive, Williamsburg, VA 23185, United States
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Alyssa Luz-Ricca
Department of Biology, Integrated Science Center, The College of William and Mary, 540 Landrum Drive, Williamsburg, VA 23185, United States
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Xida Ren
Department of Biology, Integrated Science Center, The College of William and Mary, 540 Landrum Drive, Williamsburg, VA 23185, United States
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Xingyu Zheng
Department of Biology, Integrated Science Center, The College of William and Mary, 540 Landrum Drive, Williamsburg, VA 23185, United States
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Margaret Saha
Department of Biology, Integrated Science Center, The College of William and Mary, 540 Landrum Drive, Williamsburg, VA 23185, United States
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Abstract

A fundamental principle of cellular signal processing is the encoding of information within the temporal dynamics of regulatory circuits. If synthetic circuits are to achieve the versatility and effectiveness of naturally-occurring circuits, it is necessary to develop simple, effective methods for the control of the dynamical properties of genetic circuits. However, current approaches to dynamical control often require extensive rewiring of circuit architecture, which hinders their implementation in a variety of systems. Therefore, it is essential that simple, modular, genetic parts-based frameworks are created to control the dynamical properties of circuits. Here we address this need by implementing a modular, genetic parts-based system which tunes the response time of a gene’s expression by tuning its degradation rate via the application of protein degradation tags with various affinities to their protease. This system provides a simple, easily- applicable framework for controlling the temporal aspects of genetic circuit behavior.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-ND 4.0 International license.
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Posted November 29, 2018.
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Modular, part-based control of gene expression response time using protein degradation tags
Ethan M Jones, Callan E Monette, John P Marken, Sejal Dhawan, Theresa Gibney, Christine Li, Wukun Liu, Alyssa Luz-Ricca, Xida Ren, Xingyu Zheng, Margaret Saha
bioRxiv 482331; doi: https://doi.org/10.1101/482331
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Modular, part-based control of gene expression response time using protein degradation tags
Ethan M Jones, Callan E Monette, John P Marken, Sejal Dhawan, Theresa Gibney, Christine Li, Wukun Liu, Alyssa Luz-Ricca, Xida Ren, Xingyu Zheng, Margaret Saha
bioRxiv 482331; doi: https://doi.org/10.1101/482331

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