Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Serum-dependent and independent regulation of PARP2

Qizhi Sun, View ORCID ProfileMohamed I. Gatie, View ORCID ProfileGregory M. Kelly
doi: https://doi.org/10.1101/483289
Qizhi Sun
1Department of Biology, Molecular Genetics Unit, Western University, London, ON, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Mohamed I. Gatie
1Department of Biology, Molecular Genetics Unit, Western University, London, ON, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Mohamed I. Gatie
Gregory M. Kelly
1Department of Biology, Molecular Genetics Unit, Western University, London, ON, Canada
2Department of Physiology and Pharmacology, and Paediatrics, Western University, London, ON, Canada
3Child Health Research Institute, London, ON, Canada
4Ontario Institute for Regenerative Medicine, Toronto, ON, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Gregory M. Kelly
  • For correspondence: gkelly@uwo.ca
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

PARP2 belongs to a family of proteins involved in cell differentiation, DNA damage repair, cellular energy expenditure, chromatin modeling and cell differentiation. In addition to these overlapping functions with PARP1, PARP2 participates in spermatogenesis, T-cell maturation, extraembryonic endoderm formation and adipogenesis. The function(s) of PARP2 is far from complete, and the mechanism(s) by which the gene and protein are regulated are unknown. In this study, we found that two different mechanisms are used in vitro to regulate PARP2 levels. In the presence of serum, PARP2 is degraded through the ubiquitin-proteasome pathway, however, when serum is removed, PARP2 is rapidly sequestered into an SDS- and urea-insoluble fraction. This sequestration is relieved by serum in a dose-dependent manner, and again PARP2 is detected by immunoblotting. Furthermore, and despite the presence of a putative serum response element in the PARP2 gene, transcription is not affected by serum deprivation. These observations that PARP2 is tightly regulated by distinct pathways highlights the critical roles PARP2 plays under different physiological conditions.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted November 29, 2018.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Serum-dependent and independent regulation of PARP2
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Serum-dependent and independent regulation of PARP2
Qizhi Sun, Mohamed I. Gatie, Gregory M. Kelly
bioRxiv 483289; doi: https://doi.org/10.1101/483289
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
Serum-dependent and independent regulation of PARP2
Qizhi Sun, Mohamed I. Gatie, Gregory M. Kelly
bioRxiv 483289; doi: https://doi.org/10.1101/483289

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Cell Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (4677)
  • Biochemistry (10348)
  • Bioengineering (7665)
  • Bioinformatics (26314)
  • Biophysics (13510)
  • Cancer Biology (10674)
  • Cell Biology (15427)
  • Clinical Trials (138)
  • Developmental Biology (8491)
  • Ecology (12809)
  • Epidemiology (2067)
  • Evolutionary Biology (16841)
  • Genetics (11384)
  • Genomics (15471)
  • Immunology (10606)
  • Microbiology (25190)
  • Molecular Biology (10212)
  • Neuroscience (54408)
  • Paleontology (401)
  • Pathology (1667)
  • Pharmacology and Toxicology (2892)
  • Physiology (4335)
  • Plant Biology (9239)
  • Scientific Communication and Education (1586)
  • Synthetic Biology (2557)
  • Systems Biology (6774)
  • Zoology (1461)