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optoPAD: a closed-loop optogenetics system to study the circuit basis of feeding behaviors

José-Maria Moreira, View ORCID ProfilePavel M. Itskov, View ORCID ProfileDennis Goldschmidt, View ORCID ProfileKathrin Steck, View ORCID ProfileSamuel J. Walker, View ORCID ProfileCarlos Ribeiro
doi: https://doi.org/10.1101/485110
José-Maria Moreira
Champalimaud Centre for the Unknown, Lisbon, Portugal
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Pavel M. Itskov
Champalimaud Centre for the Unknown, Lisbon, Portugal
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Dennis Goldschmidt
Champalimaud Centre for the Unknown, Lisbon, Portugal
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Kathrin Steck
Champalimaud Centre for the Unknown, Lisbon, Portugal
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Samuel J. Walker
Champalimaud Centre for the Unknown, Lisbon, Portugal
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Carlos Ribeiro
Champalimaud Centre for the Unknown, Lisbon, Portugal
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  • For correspondence: Carlos.Ribeiro@neuro.fchampalimaud.org
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Abstract

The regulation of feeding plays a key role in determining the fitness of animals through its impact on nutrition. Elucidating the circuit basis of feeding and related behaviors is an important goal in neuroscience. We recently used a system for closed-loop optogenetic manipulation of neurons contingent on the fly’s feeding behavior to dissect the impact of a specific subset of taste neurons on yeast feeding (Steck et al., 2018). Here we describe the development and validation of this system, which we term the optoPAD. We use the optoPAD to induce appetitive and aversive effects on feeding by activating or inhibiting gustatory neurons in closed loop – effectively creating virtual taste realities. The use of optogenetics allowed us to vary the dynamics and probability of stimulation in single flies and assess the impact on feeding behavior quantitatively and with high throughput. These data demonstrate that the optoPAD is a powerful tool to dissect the circuit basis of feeding behavior, allowing the efficient implementation of sophisticated behavioral paradigms to study the mechanistic basis of animals’ adaptation to dynamic environments.

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Posted December 03, 2018.
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optoPAD: a closed-loop optogenetics system to study the circuit basis of feeding behaviors
José-Maria Moreira, Pavel M. Itskov, Dennis Goldschmidt, Kathrin Steck, Samuel J. Walker, Carlos Ribeiro
bioRxiv 485110; doi: https://doi.org/10.1101/485110
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optoPAD: a closed-loop optogenetics system to study the circuit basis of feeding behaviors
José-Maria Moreira, Pavel M. Itskov, Dennis Goldschmidt, Kathrin Steck, Samuel J. Walker, Carlos Ribeiro
bioRxiv 485110; doi: https://doi.org/10.1101/485110

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