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The STROBE: a system for closed-looped optogenetic control of freely feeding flies

Pierre-Yves Musso, Pierre Junca, Meghan Jelen, Damian Feldman-Kiss, Han Zhang, Rachel C.W. Chan, View ORCID ProfileMichael D. Gordon
doi: https://doi.org/10.1101/486050
Pierre-Yves Musso
1Department of Zoology, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
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Pierre Junca
1Department of Zoology, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
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Meghan Jelen
1Department of Zoology, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
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Damian Feldman-Kiss
1Department of Zoology, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
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Han Zhang
2Engineering Physics Program, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
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Rachel C.W. Chan
2Engineering Physics Program, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
3Present address: Department of Computer Science, University of Toronto, Toronto, ON M5S 3G4.
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Michael D. Gordon
1Department of Zoology, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
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  • ORCID record for Michael D. Gordon
  • For correspondence: gordon@zoology.ubc.ca
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Abstract

Manipulating feeding circuits in freely moving animals is challenging, in part because the timing of sensory inputs is affected by the animal’s behavior. To address this challenge in Drosophila, we developed the Sip-Triggered Optogenetic Behavior Enclosure (“STROBE”). The STROBE is a closed-looped system for real-time optogenetic activation of feeding flies, designed to evoke neural excitation coincident with food contact. We demonstrate that optogenetic stimulation of sweet sensory neurons in the STROBE drives attraction to tasteless food, while activation of bitter sensory neurons promotes avoidance. Moreover, feeding behavior in the STROBE is modified by the fly’s internal state, as well as the presence of chemical taste ligands. We also find that mushroom body dopaminergic neurons and their respective post-synaptic partners drive opposing feeding behaviors following activation. Together, these results establish the STROBE as a new tool for dissecting fly feeding circuits and suggest a role for mushroom body circuits in processing naïve taste responses.

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Posted December 04, 2018.
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The STROBE: a system for closed-looped optogenetic control of freely feeding flies
Pierre-Yves Musso, Pierre Junca, Meghan Jelen, Damian Feldman-Kiss, Han Zhang, Rachel C.W. Chan, Michael D. Gordon
bioRxiv 486050; doi: https://doi.org/10.1101/486050
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The STROBE: a system for closed-looped optogenetic control of freely feeding flies
Pierre-Yves Musso, Pierre Junca, Meghan Jelen, Damian Feldman-Kiss, Han Zhang, Rachel C.W. Chan, Michael D. Gordon
bioRxiv 486050; doi: https://doi.org/10.1101/486050

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