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A quantitative inventory of yeast P body proteins reveals principles of compositional specificity

Wenmin Xing, Denise Muhlrad, Roy Parker, Michael K. Rosen
doi: https://doi.org/10.1101/489658
Wenmin Xing
1Department of Biophysics, Howard Hughes Medical Institute, UT Southwestern Medical Center, Dallas, TX, 79390
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Denise Muhlrad
2Department of Biochemistry, Howard Hughes Medical Institute, University of Colorado, Boulder, CO, 80309
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Roy Parker
2Department of Biochemistry, Howard Hughes Medical Institute, University of Colorado, Boulder, CO, 80309
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Michael K. Rosen
1Department of Biophysics, Howard Hughes Medical Institute, UT Southwestern Medical Center, Dallas, TX, 79390
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ABSTRACT

P bodies are archetypal biomolecular condensates that concentrate proteins and RNA without a surrounding membrane. While dozens of P body proteins are known, the concentrations of components in the compartment have not been measured. We used live cell imaging to generate a quantitative inventory of the major proteins in yeast P bodies. Only 7 proteins are highly concentrated in P bodies (5.1-15 μM); the 24 others examined are appreciably lower (most ≤ 2.4 μM). P body concentration correlates inversely with cytoplasmic exchange rate. Sequence elements driving Dcp2 concentration into P bodies are distributed across the protein and act cooperatively. Strong correlation between interaction valency of components and P body concentration suggests how the composition of other condensates may be estimated from known interaction data. Our data indicate that P bodies, and probably other condensates, are biochemically simpler than suggested by proteomic analyses, with implications for specificity, reconstitution and evolution.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted December 07, 2018.
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A quantitative inventory of yeast P body proteins reveals principles of compositional specificity
Wenmin Xing, Denise Muhlrad, Roy Parker, Michael K. Rosen
bioRxiv 489658; doi: https://doi.org/10.1101/489658
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A quantitative inventory of yeast P body proteins reveals principles of compositional specificity
Wenmin Xing, Denise Muhlrad, Roy Parker, Michael K. Rosen
bioRxiv 489658; doi: https://doi.org/10.1101/489658

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