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Embryo geometry drives formation of robust signaling gradients through receptor localization

Zhechun Zhang, Steven Zwick, Ethan Loew, Joshua S. Grimley, Sharad Ramanathan
doi: https://doi.org/10.1101/491290
Zhechun Zhang
1Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138
2School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138
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  • For correspondence: zhechunzhang@fas.harvard.edu sharad@post.harvard.edu
Steven Zwick
2School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138
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Ethan Loew
1Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138
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Joshua S. Grimley
3Allen Institute for Brain Science, Seattle, WA 98109
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Sharad Ramanathan
1Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138
2School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138
4Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02138
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  • For correspondence: zhechunzhang@fas.harvard.edu sharad@post.harvard.edu
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Abstract

Morphogen signals are essential for cell fate specification during embryogenesis. Some receptors that sense these morphogen signals are known to localize to only the apical or basal membrane of polarized cell lines in vitro. How such localization affects morphogen sensing and patterning in the developing embryo remains unknown. Here, we show in the early mouse embryo that the formation of a robust BMP signaling gradient depends on restricted, basolateral localization of the BMP receptors. Mis-localizing these receptors to apical membrane leads to ectopic BMP signaling in vivo in the mouse embryo. To reach the basolaterally localized receptors in epiblast, BMP4 ligand, secreted by the extra-embryonic ectoderm, has to diffuses through the narrow interstitial space between the epiblast and the underlying endoderm. This restricted, basolateral diffusion creates a signaling gradient. The embryo geometry further buffers the gradient from fluctuations in the levels of BMP4. Our results demonstrate the importance of receptor localization and embryo geometry in shaping morphogen signaling during embryogenesis.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted December 11, 2018.
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Embryo geometry drives formation of robust signaling gradients through receptor localization
Zhechun Zhang, Steven Zwick, Ethan Loew, Joshua S. Grimley, Sharad Ramanathan
bioRxiv 491290; doi: https://doi.org/10.1101/491290
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Embryo geometry drives formation of robust signaling gradients through receptor localization
Zhechun Zhang, Steven Zwick, Ethan Loew, Joshua S. Grimley, Sharad Ramanathan
bioRxiv 491290; doi: https://doi.org/10.1101/491290

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