Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Quantification of microenvironmental metabolites in murine cancer models reveals determinants of tumor nutrient availability

Mark R. Sullivan, Laura V. Danai, Caroline A. Lewis, Sze Ham Chan, Dan Y. Gui, Tenzin Kunchok, Emily A. Dennstedt, Matthew G. Vander Heiden, Alexander Muir
doi: https://doi.org/10.1101/492652
Mark R. Sullivan
1Koch Institute for Integrative Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Laura V. Danai
1Koch Institute for Integrative Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
2Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, Amherst, MA 01003, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Caroline A. Lewis
3Department of Biology and Whitehead Institute for Biomedical Research and Massachusetts Institute of Technology, Cambridge, MA 02142, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sze Ham Chan
3Department of Biology and Whitehead Institute for Biomedical Research and Massachusetts Institute of Technology, Cambridge, MA 02142, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Dan Y. Gui
1Koch Institute for Integrative Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Tenzin Kunchok
3Department of Biology and Whitehead Institute for Biomedical Research and Massachusetts Institute of Technology, Cambridge, MA 02142, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Emily A. Dennstedt
1Koch Institute for Integrative Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Matthew G. Vander Heiden
1Koch Institute for Integrative Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
4Dana-Farber Cancer Institute, Boston, MA 02115, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: amuir@uchicago.edu mvh@mit.edu
Alexander Muir
1Koch Institute for Integrative Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
5Ben May Department for Cancer Research, University of Chicago, Chicago, IL 60637, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: amuir@uchicago.edu mvh@mit.edu
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

Cancer cell metabolism is heavily influenced by microenvironmental factors, including nutrient availability. Therefore, knowledge of microenvironmental nutrient levels is essential to understand tumor metabolism. To measure the extracellular nutrient levels available to tumors, we developed a quantitative metabolomics method to measure the absolute concentrations of >118 metabolites in plasma and tumor interstitial fluid, the extracellular fluid that perfuses tumors. Comparison of nutrient levels in tumor interstitial fluid and plasma revealed that the nutrients available to tumors differ from those present in circulation. Further, by comparing interstitial fluid nutrient levels between autochthonous and transplant models of murine pancreatic and lung adenocarcinoma, we found that tumor type, anatomical location and animal diet affect local nutrient availability. These data provide a comprehensive characterization of the nutrients present in the tumor microenvironment of widely used models of lung and pancreatic cancer and identify factors that influence metabolite levels in tumors.

Impact Statement Nutrient availability is an important tumor microenvironmental factor that impacts cancer cell biology; we developed methods to measure nutrients available to tumor cells and characterized factors that influence tumor nutrient availability.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
Back to top
PreviousNext
Posted December 10, 2018.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Quantification of microenvironmental metabolites in murine cancer models reveals determinants of tumor nutrient availability
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Quantification of microenvironmental metabolites in murine cancer models reveals determinants of tumor nutrient availability
Mark R. Sullivan, Laura V. Danai, Caroline A. Lewis, Sze Ham Chan, Dan Y. Gui, Tenzin Kunchok, Emily A. Dennstedt, Matthew G. Vander Heiden, Alexander Muir
bioRxiv 492652; doi: https://doi.org/10.1101/492652
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
Quantification of microenvironmental metabolites in murine cancer models reveals determinants of tumor nutrient availability
Mark R. Sullivan, Laura V. Danai, Caroline A. Lewis, Sze Ham Chan, Dan Y. Gui, Tenzin Kunchok, Emily A. Dennstedt, Matthew G. Vander Heiden, Alexander Muir
bioRxiv 492652; doi: https://doi.org/10.1101/492652

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Cancer Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (4222)
  • Biochemistry (9096)
  • Bioengineering (6744)
  • Bioinformatics (23927)
  • Biophysics (12077)
  • Cancer Biology (9485)
  • Cell Biology (13722)
  • Clinical Trials (138)
  • Developmental Biology (7614)
  • Ecology (11652)
  • Epidemiology (2066)
  • Evolutionary Biology (15469)
  • Genetics (10613)
  • Genomics (14289)
  • Immunology (9453)
  • Microbiology (22759)
  • Molecular Biology (9057)
  • Neuroscience (48818)
  • Paleontology (354)
  • Pathology (1479)
  • Pharmacology and Toxicology (2560)
  • Physiology (3820)
  • Plant Biology (8307)
  • Scientific Communication and Education (1467)
  • Synthetic Biology (2285)
  • Systems Biology (6168)
  • Zoology (1297)