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Satellite DNA-containing gigantic introns in a unique gene expression program during Drosophila spermatogenesis

Jaclyn M Fingerhut, Jessica V. Moran, View ORCID ProfileYukiko M Yamashita
doi: https://doi.org/10.1101/493254
Jaclyn M Fingerhut
1Cellular and Molecular Biology Program, University of Michigan, Ann Arbor, MI 48109, USA
2Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109, USA
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Jessica V. Moran
2Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109, USA
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Yukiko M Yamashita
1Cellular and Molecular Biology Program, University of Michigan, Ann Arbor, MI 48109, USA
2Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109, USA
3Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA
4Howard Hughes Medical Institute, University of Michigan, Ann Arbor, MI 48109, USA
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  • ORCID record for Yukiko M Yamashita
  • For correspondence: yukikomy@umich.edu
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Abstract

Intron gigantism, where genes contain megabase-sized introns, is observed across species, yet little is known about its purpose or regulation. Here we identify a unique gene expression program utilized for the proper expression of genes with intron gigantism. We find that two Drosophila genes with intron gigantism, kl-3 and kl-5, are transcribed in a spatiotemporal manner over the course of spermatocyte differentiation, which spans ~90 hours. The introns of these genes contain megabases of simple satellite DNA repeats that comprise over 99% of the gene loci, and these satellite-DNA containing introns are transcribed. We identify two RNA-binding proteins that specifically localize to kl-3 and kl-5 transcripts and are needed for the successful transcription or processing of these genes. We propose that genes with intron gigantism require a unique gene expression program, which may serve as a platform to regulate gene expression during cellular differentiation.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted December 11, 2018.
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Satellite DNA-containing gigantic introns in a unique gene expression program during Drosophila spermatogenesis
Jaclyn M Fingerhut, Jessica V. Moran, Yukiko M Yamashita
bioRxiv 493254; doi: https://doi.org/10.1101/493254
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Satellite DNA-containing gigantic introns in a unique gene expression program during Drosophila spermatogenesis
Jaclyn M Fingerhut, Jessica V. Moran, Yukiko M Yamashita
bioRxiv 493254; doi: https://doi.org/10.1101/493254

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