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Uncultured marine cyanophages encode for active NblA, phycobilisome proteolysis adaptor protein

Omer Nadel, Andrey Rozenberg, José Flores-Uribe, Shirley Larom, Rakefet Schwarz, View ORCID ProfileOded Béjà
doi: https://doi.org/10.1101/494369
Omer Nadel
1Faculty of Biology, Technion - Israel Institute of Technology, Haifa 32000, Israel
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Andrey Rozenberg
1Faculty of Biology, Technion - Israel Institute of Technology, Haifa 32000, Israel
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José Flores-Uribe
1Faculty of Biology, Technion - Israel Institute of Technology, Haifa 32000, Israel
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Shirley Larom
1Faculty of Biology, Technion - Israel Institute of Technology, Haifa 32000, Israel
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Rakefet Schwarz
2The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 5290002, Israel
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Oded Béjà
1Faculty of Biology, Technion - Israel Institute of Technology, Haifa 32000, Israel
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  • ORCID record for Oded Béjà
  • For correspondence: beja@technion.ac.il
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Summary

Phycobilisomes (PBS) are large water-soluble membrane-associated complexes in cyanobacteria and some chloroplasts that serve as a light-harvesting antennas for the photosynthetic apparatus. When short of nitrogen or sulfur, cyanobacteria readily degrade their phycobilisomes allowing the cell to replenish the vanishing nutrients. The key regulator in the degradation process is NblA, a small protein (~6 kDa) which recruits proteases to the PBS. It was discovered previously that not only do cyanobacteria possess nblA genes but also that they are encoded by genomes of some freshwater cyanophages. A recent study, using assemblies from oceanic metagenomes, revealed genomes of a novel uncultured marine cyanophage lineage which contain genes coding for the PBS degradation protein. Here, we examine the functionality of nblA-like genes from these marine cyanophages by testing them in a freshwater model cyanobacterial nblA knockout. One of the viral NblA variants could complement the non-bleaching phenotype and restore PBS degradation. Our findings reveal a functional NblA from a novel marine cyanophage lineage. Furthermore, we shed new light on the distribution of nblA genes in cyanobacteria and cyanophages.

Originality-Significance Statement This is the first study to examine the distribution and function of nblA genes of marine cyanophage origin. We describe as well the distribution of nblA-like genes in marine cyanobacteria using bioinformatic methods.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted December 13, 2018.
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Uncultured marine cyanophages encode for active NblA, phycobilisome proteolysis adaptor protein
Omer Nadel, Andrey Rozenberg, José Flores-Uribe, Shirley Larom, Rakefet Schwarz, Oded Béjà
bioRxiv 494369; doi: https://doi.org/10.1101/494369
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Uncultured marine cyanophages encode for active NblA, phycobilisome proteolysis adaptor protein
Omer Nadel, Andrey Rozenberg, José Flores-Uribe, Shirley Larom, Rakefet Schwarz, Oded Béjà
bioRxiv 494369; doi: https://doi.org/10.1101/494369

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