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Rapid Determination of Antibiotic Susceptibility by Stimulated Raman Scattering Imaging of D2O Metabolism

Weili Hong, Ji-Xin Cheng
doi: https://doi.org/10.1101/496778
Weili Hong
†Beijing Advanced Innovation Center for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang University, Beijing 100083, China
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Ji-Xin Cheng
‡Department of Electrical & Computer Engineering, Massachusetts 02215, United States
§Department of Biomedical Engineering, Boston University, Boston, Massachusetts 02215, United States
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  • For correspondence: jxcheng@bu.edu
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Abstract

Rapid antibiotic susceptibility testing (AST) is urgently needed for treating infections with correct antibiotics and slowing down the emergence of antibiotic resistant bacteria. Current clinical methods reply on culture and take at least 16 h. Here, using P. aeruginosa, E. coli and S. aureus as models, we show that the AST can be finished in 10 minutes by stimulated Raman scattering (SRS) imaging of D2O metabolic activities. The metabolic incorporation of D2O, which is used for biomolecule synthesis, can be monitored in a single bacterium. Time lapse experiments show that the C-D vibrational signal can be observed in a single bacterium within 10 minutes culture in D2O medium. Since water is universally used for biosynthesis in bacteria, SRS imaging of D2O metabolism has the potential to be generalizable to different bacteria species.

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Posted December 14, 2018.
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Rapid Determination of Antibiotic Susceptibility by Stimulated Raman Scattering Imaging of D2O Metabolism
Weili Hong, Ji-Xin Cheng
bioRxiv 496778; doi: https://doi.org/10.1101/496778
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Rapid Determination of Antibiotic Susceptibility by Stimulated Raman Scattering Imaging of D2O Metabolism
Weili Hong, Ji-Xin Cheng
bioRxiv 496778; doi: https://doi.org/10.1101/496778

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