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Revisiting the window of opportunity for co-transcriptional splicing efficiency and fidelity

Vahid Aslanzadeh, View ORCID ProfileJean D. Beggs
doi: https://doi.org/10.1101/497040
Vahid Aslanzadeh
Wellcome Centre for Cell Biology, University of Edinburgh, Michael Swann Building, Kings Buildings, Mayfield Road, Edinburgh EH9 3BF, UKCentre for Cardiovascular Science, Queen’s Medical Research Institute, University of Edinburgh, Little France Crescent, Edinburgh EH16 4TJ, UK
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Jean D. Beggs
Wellcome Centre for Cell Biology, University of Edinburgh, Michael Swann Building, Kings Buildings, Mayfield Road, Edinburgh EH9 3BF, UK
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  • ORCID record for Jean D. Beggs
  • For correspondence: jbeggs@ed.ac.uk
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Abstract

Recently, we reported that changes in transcription elongation rate affect the efficiency and fidelity of precursor mRNA (pre-mRNA) splicing, especially of ribosomal protein (RP) transcripts. Here, we analyse these results in more detail, finding that the majority of RP transcripts with non-consensus 5’ splice sites have reduced splicing efficiency with faster transcription elongation, and improved efficiency with slower elongation, as might be predicted by the “window of opportunity” model for co-transcriptional splicing. In contrast, both faster and slower elongation reduce splicing fidelity, often for the same splicing events, and both faster and slower transcription increase fidelity with a different set of splicing events. We propose that certain non-consensus 5’ splice sites in ribosomal protein transcripts confer a stronger effect of transcription elongation rate on splicing efficiency, possibly by causing a rate-limiting step that delays activation of spliceosomes. The effects of different rates of transcription elongation on splicing fidelity are more difficult to explain by a simple window of opportunity model. We discuss these new findings in the context of current models of co-transcriptional splicing and splicing fidelity.

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Posted December 16, 2018.
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Revisiting the window of opportunity for co-transcriptional splicing efficiency and fidelity
Vahid Aslanzadeh, Jean D. Beggs
bioRxiv 497040; doi: https://doi.org/10.1101/497040
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Revisiting the window of opportunity for co-transcriptional splicing efficiency and fidelity
Vahid Aslanzadeh, Jean D. Beggs
bioRxiv 497040; doi: https://doi.org/10.1101/497040

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