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A novel mechanosensitive channel controls osmoregulation, differentiation and infectivity in Trypanosoma cruzi

N Dave, U Cetiner, D Arroyo, J Fonbuena, M Tiwari, P Barrera, N Lander, A Anishkin, S Sukharev, View ORCID ProfileV Jimenez
doi: https://doi.org/10.1101/498469
N Dave
1Department of Biological Science, College of Natural Sciences and Mathematics, California State University Fullerton
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U Cetiner
2Department of Biology, University of Maryland, College Park
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D Arroyo
1Department of Biological Science, College of Natural Sciences and Mathematics, California State University Fullerton
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J Fonbuena
1Department of Biological Science, College of Natural Sciences and Mathematics, California State University Fullerton
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M Tiwari
1Department of Biological Science, College of Natural Sciences and Mathematics, California State University Fullerton
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P Barrera
3Departmento de Biología, Facultad de Ciencias Exactas y Naturales, Instituto de Histologia y Embriologia IHEM-CONICET, Facultad de Medicina, Universidad Nacional de Cuyo
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N Lander
4Department of Biological Sciences, University of Cincinnati, Cincinnati, OH 45221
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A Anishkin
2Department of Biology, University of Maryland, College Park
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S Sukharev
2Department of Biology, University of Maryland, College Park
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V Jimenez
1Department of Biological Science, College of Natural Sciences and Mathematics, California State University Fullerton
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  • ORCID record for V Jimenez
  • For correspondence: vjimenezortiz@fullerton.edu
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Abstract

Trypanosoma cruzi, the causative agent of Chagas disease, undergoes drastic morphological and biochemical modifications as it passes between hosts and transitions from extracellular to intracellular stages. The osmotic and mechanical aspects of these cellular transformations are not understood. Here we identify and characterize a novel mechanosensitive channel in T. cruzi (TcMscS) belonging to the superfamily of small conductance mechanosensitive channels (MscS). TcMscS is activated by membrane tension and forms a large pore permeable to anions, cations, and small osmolytes. The channel changes its location from the contractile vacuole complex in epimastigotes to the plasma membrane as the parasites develop into intracellular amastigotes. TcMscS knockout parasites show significant fitness defects, including increased cell volume, calcium dysregulation, impaired differentiation, and a dramatic decrease in infectivity. Our work provides mechanistic insights into components supporting pathogen adaptation inside the host thus opening the exploration of mechanosensation as a prerequisite of protozoan infectivity.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • Figures 1, 2, 5, 6 and 8 have been modified, Figure 7 contains new data, supplementary information has been reorganized and new methods incorporated.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted February 19, 2021.
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A novel mechanosensitive channel controls osmoregulation, differentiation and infectivity in Trypanosoma cruzi
N Dave, U Cetiner, D Arroyo, J Fonbuena, M Tiwari, P Barrera, N Lander, A Anishkin, S Sukharev, V Jimenez
bioRxiv 498469; doi: https://doi.org/10.1101/498469
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A novel mechanosensitive channel controls osmoregulation, differentiation and infectivity in Trypanosoma cruzi
N Dave, U Cetiner, D Arroyo, J Fonbuena, M Tiwari, P Barrera, N Lander, A Anishkin, S Sukharev, V Jimenez
bioRxiv 498469; doi: https://doi.org/10.1101/498469

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