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Evolutionary implications of the first microRNA- and piRNA complement of Lepidodermella squamata (Gastrotricha)

View ORCID ProfileBastian Fromm, Juan Pablo Tosar, Felipe Aguilera, View ORCID ProfileMarc R. Friedländer, Lutz Bachmann, View ORCID ProfileAndreas Hejnol
doi: https://doi.org/10.1101/504100
Bastian Fromm
1Science for Life Laboratory, Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, Stockholm, Sweden
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  • For correspondence: Bastian.Fromm@scilifelab.se
Juan Pablo Tosar
2Functional Genomics Unit, Institut Pasteur de Montevideo, Montevideo, Uruguay
3Nuclear Research Center, Faculty of Science, Universidad de la República, Montevideo, Uruguay
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Felipe Aguilera
4Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción, Chile
5Sars International Centre for Marine Molecular Biology, University of Bergen, Bergen, Norway
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Marc R. Friedländer
1Science for Life Laboratory, Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, Stockholm, Sweden
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Lutz Bachmann
6Research group Frontiers in Evolutionary Zoology, Natural History Museum, University of Oslo, Oslo, Norway
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Andreas Hejnol
5Sars International Centre for Marine Molecular Biology, University of Bergen, Bergen, Norway
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Abstract

Gastrotrichs - hairy bellies - are microscopic free-living animals inhabiting marine and freshwater habitats. Based on morphological and early molecular analyses, gastrotrichs were placed close to nematodes, but recent phylogenomic analyses have suggested their close relationship to flatworms (Platyhelminthes) within Spiralia. Small non-coding RNA data on e.g. microRNAs (miRNAs) and PIWI-interacting RNAs (piRNA) may help to resolve this long-standing question. MiRNAs are short post-transcriptional gene regulators that together with piRNAs play key roles in development. In a ‘multi-omics’ approach we here used small-RNA sequencing, available transcriptome and genomic data to unravel the miRNA-and piRNA complements along with the RNAi protein machinery of Lepidodermella squamata (Gastrotricha, Chaetonotida). We identified 52 miRNA genes representing 35 highly conserved miRNA families specific to Eumetazoa, Bilateria, Protostomia, and Spiralia, respectively, with overall high similarities to platyhelminth miRNA complements. In addition, we found four large piRNA clusters that also resemble flatworm piRNAs but not those earlier described for nematodes. Congruently, transcriptomic annotation revealed that the Lepidodermella protein machinery is highly similar to flatworms, too. Taken together, miRNA, piRNA and protein data support a close relationship of gastrotrichs and flatworms.

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Posted January 04, 2019.
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Evolutionary implications of the first microRNA- and piRNA complement of Lepidodermella squamata (Gastrotricha)
Bastian Fromm, Juan Pablo Tosar, Felipe Aguilera, Marc R. Friedländer, Lutz Bachmann, Andreas Hejnol
bioRxiv 504100; doi: https://doi.org/10.1101/504100
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Evolutionary implications of the first microRNA- and piRNA complement of Lepidodermella squamata (Gastrotricha)
Bastian Fromm, Juan Pablo Tosar, Felipe Aguilera, Marc R. Friedländer, Lutz Bachmann, Andreas Hejnol
bioRxiv 504100; doi: https://doi.org/10.1101/504100

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