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Neocortical layer 4 in adult mouse differs in major cell types and circuit organization between primary sensory areas

F. Scala, View ORCID ProfileD. Kobak, S. Shan, Y. Bernaerts, S. Laturnus, View ORCID ProfileC.R. Cadwell, L. Hartmanis, E. Froudarakis, J. Castro, Z.H. Tan, S. Papadopoulos, S. Patel, View ORCID ProfileR. Sandberg, View ORCID ProfileP. Berens, View ORCID ProfileX. Jiang, View ORCID ProfileA.S. Tolias
doi: https://doi.org/10.1101/507293
F. Scala
1Department of Neuroscience, Baylor College of Medicine, Houston, TX, USA
2Center for Neuroscience and Artificial Intelligence, Baylor College of Medicine, Houston, Texas, USA
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D. Kobak
3Institute for Ophthalmic Research, University of Tübingen, Tübingen, Germany
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  • ORCID record for D. Kobak
S. Shan
1Department of Neuroscience, Baylor College of Medicine, Houston, TX, USA
2Center for Neuroscience and Artificial Intelligence, Baylor College of Medicine, Houston, Texas, USA
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Y. Bernaerts
3Institute for Ophthalmic Research, University of Tübingen, Tübingen, Germany
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S. Laturnus
3Institute for Ophthalmic Research, University of Tübingen, Tübingen, Germany
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C.R. Cadwell
4Department of Anatomic Pathology, University of California San Francisco, San Francisco, CA, USA
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L. Hartmanis
5Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden
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E. Froudarakis
1Department of Neuroscience, Baylor College of Medicine, Houston, TX, USA
2Center for Neuroscience and Artificial Intelligence, Baylor College of Medicine, Houston, Texas, USA
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J. Castro
1Department of Neuroscience, Baylor College of Medicine, Houston, TX, USA
2Center for Neuroscience and Artificial Intelligence, Baylor College of Medicine, Houston, Texas, USA
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Z.H. Tan
1Department of Neuroscience, Baylor College of Medicine, Houston, TX, USA
2Center for Neuroscience and Artificial Intelligence, Baylor College of Medicine, Houston, Texas, USA
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S. Papadopoulos
1Department of Neuroscience, Baylor College of Medicine, Houston, TX, USA
2Center for Neuroscience and Artificial Intelligence, Baylor College of Medicine, Houston, Texas, USA
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S. Patel
1Department of Neuroscience, Baylor College of Medicine, Houston, TX, USA
2Center for Neuroscience and Artificial Intelligence, Baylor College of Medicine, Houston, Texas, USA
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R. Sandberg
5Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden
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P. Berens
3Institute for Ophthalmic Research, University of Tübingen, Tübingen, Germany
6Department of Computer Science, University of Tübingen, Tübingen, Germany
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X. Jiang
1Department of Neuroscience, Baylor College of Medicine, Houston, TX, USA
2Center for Neuroscience and Artificial Intelligence, Baylor College of Medicine, Houston, Texas, USA
7Jan and Dan Duncan Neurological Research Institute at Texas Children’s Hospital, Houston, Texas, USA
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  • For correspondence: astolias@bcm.edu xiaolonj@bcm.edu
A.S. Tolias
1Department of Neuroscience, Baylor College of Medicine, Houston, TX, USA
2Center for Neuroscience and Artificial Intelligence, Baylor College of Medicine, Houston, Texas, USA
8Department of Electrical and Computational Engineering, Rice University, Houston, Texas, USA
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  • For correspondence: astolias@bcm.edu xiaolonj@bcm.edu
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Abstract

Layer 4 (L4) of mammalian neocortex plays a crucial role in cortical information processing, yet a complete census of its cell types and connectivity remains elusive. Using whole-cell recordings with morphological recovery, we identified one major excitatory and seven inhibitory types of neurons in L4 of adult mouse visual cortex (V1). Nearly all excitatory neurons were pyramidal and all somatostatin-positive (SOM+) non-fast-spiking neurons were Martinotti cells. In contrast, in somatosensory cortex (S1), excitatory neurons were mostly stellate and SOM+ neurons were non-Martinotti. These morphologically distinct SOM+ interneurons corresponded to different transcriptomic cell types and were differentially integrated into the local circuit with only S1 neurons receiving local excitatory input. We propose that cell-type specific circuit motifs, such as the Martinotti/pyramidal and non-Martinotti/stellate pairs, are optionally used across the cortex as building blocks to assemble cortical circuits.

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Posted April 11, 2019.
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Neocortical layer 4 in adult mouse differs in major cell types and circuit organization between primary sensory areas
F. Scala, D. Kobak, S. Shan, Y. Bernaerts, S. Laturnus, C.R. Cadwell, L. Hartmanis, E. Froudarakis, J. Castro, Z.H. Tan, S. Papadopoulos, S. Patel, R. Sandberg, P. Berens, X. Jiang, A.S. Tolias
bioRxiv 507293; doi: https://doi.org/10.1101/507293
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Neocortical layer 4 in adult mouse differs in major cell types and circuit organization between primary sensory areas
F. Scala, D. Kobak, S. Shan, Y. Bernaerts, S. Laturnus, C.R. Cadwell, L. Hartmanis, E. Froudarakis, J. Castro, Z.H. Tan, S. Papadopoulos, S. Patel, R. Sandberg, P. Berens, X. Jiang, A.S. Tolias
bioRxiv 507293; doi: https://doi.org/10.1101/507293

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