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Differential expression of BMP antagonists, gremlin and noggin in hydra: antagonism between Wnt and BMP pathways

View ORCID ProfileLakshmi Surekha Krishnapati, View ORCID ProfileSamiksha Khade, View ORCID ProfileDiptee Trimbake, View ORCID ProfileRohan Patwardhan, View ORCID ProfileSiva Kumar Nadimpalli, View ORCID ProfileSurendra Ghaskadbi
doi: https://doi.org/10.1101/510602
Lakshmi Surekha Krishnapati
Developmental Biology Group, MACS-Agharkar Research Institute, Pune-411004, INDIA;
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  • ORCID record for Lakshmi Surekha Krishnapati
Samiksha Khade
Developmental Biology Group, MACS-Agharkar Research Institute, Pune-411004, INDIA;
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Diptee Trimbake
Developmental Biology Group, MACS-Agharkar Research Institute, Pune-411004, INDIA;
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Rohan Patwardhan
Developmental Biology Group, MACS-Agharkar Research Institute, Pune-411004, INDIA;
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Siva Kumar Nadimpalli
Laboratory for Protein Biochemistry and Glycobiology, Biochemistry Department, University of Hydera
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Surendra Ghaskadbi
Developmental Biology Group, MACS-Agharkar Research Institute, Pune-411004, INDIA;
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  • For correspondence: ghaskadbi@gmail.com
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Abstract

Mechanisms regulating BMP and Wnt signaling pathways have been widely studied in many organisms. One of the mechanisms by which these pathways are regulated is by binding of extracellular ligands. In the present study, we report studies with two BMP antagonists, gremlin and noggin from Hydra vulgaris Ind-Pune and demonstrate antagonistic relationship between BMP and Wnt pathways. Gremlin was ubiquitously expressed from the body column to head region except in the basal disc and hypostome. During budding, gremlin was expressed predominantly in the budding region suggesting a possible role in budding; this was confirmed in polyps with different stages of buds. Noggin, on the other hand, was predominantly expressed in the endoderm of hypostome, base of the tentacles, lower body column and at the basal disc in whole polyps. During budding, noggin was expressed at the sites of emergence of tentacles suggesting a role in tentacle formation. This was confirmed in alsterpaullone-treated polyps, which showed noggin expression as distinct spots where ectopic organizers and ectopic tentacles eventually formed. Using RT-PCR, we found that up-regulation of Wnt is accompanied with down-regulation of BMP5-8b demonstrating antagonism between the two pathways. Down-regulation of noggin and gremlin, however, occurred only after 24 h recovery. The data suggest that inhibition of BMP pathway by Wnt signaling in hydra does not directly involve noggin and gremlin. Our findings indicate that the BMP/Noggin antagonism evolved early for setting up and/or maintaining the head organizer while involvement of these BMP antagonists during vertebrate axial patterning are recent evolutionary acquisitions.

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Posted January 03, 2019.
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Differential expression of BMP antagonists, gremlin and noggin in hydra: antagonism between Wnt and BMP pathways
Lakshmi Surekha Krishnapati, Samiksha Khade, Diptee Trimbake, Rohan Patwardhan, Siva Kumar Nadimpalli, Surendra Ghaskadbi
bioRxiv 510602; doi: https://doi.org/10.1101/510602
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Differential expression of BMP antagonists, gremlin and noggin in hydra: antagonism between Wnt and BMP pathways
Lakshmi Surekha Krishnapati, Samiksha Khade, Diptee Trimbake, Rohan Patwardhan, Siva Kumar Nadimpalli, Surendra Ghaskadbi
bioRxiv 510602; doi: https://doi.org/10.1101/510602

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