Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Buckling of epithelium growing under spherical confinement

Anastasiya Trushko, Ilaria Di Meglio, Aziza Merzouki, Carles Blanch-Mercader, Shada Abuhattum, Jochen Guck, Kevin Alessandri, Pierre Nassoy, Karsten Kruse, Bastien Chopard, View ORCID ProfileAurélien Roux
doi: https://doi.org/10.1101/513119
Anastasiya Trushko
1department of Biochemistry, University of Geneva, CH-1211 Geneva, Switzerland.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ilaria Di Meglio
1department of Biochemistry, University of Geneva, CH-1211 Geneva, Switzerland.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Aziza Merzouki
2department of Computer Science, University of Geneva, CH-1211 Geneva, Switzerland.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Carles Blanch-Mercader
1department of Biochemistry, University of Geneva, CH-1211 Geneva, Switzerland.
3department of Theoretical Physics, University of Geneva, CH-1211 Geneva, Switzerland.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Shada Abuhattum
4Biotechnology Center, Technische Universität Dresden, D-01307 Dresden, Germany.
5JPK Instruments AG, 12099 Berlin, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jochen Guck
4Biotechnology Center, Technische Universität Dresden, D-01307 Dresden, Germany.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kevin Alessandri
6Université de Bordeaux, Laboratoire Photonique Numérique et Nanosciences & CNRS and Institut d’Optique, LP2N, UMR 5298, F-33400, Talence, France.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Pierre Nassoy
6Université de Bordeaux, Laboratoire Photonique Numérique et Nanosciences & CNRS and Institut d’Optique, LP2N, UMR 5298, F-33400, Talence, France.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Karsten Kruse
1department of Biochemistry, University of Geneva, CH-1211 Geneva, Switzerland.
3department of Theoretical Physics, University of Geneva, CH-1211 Geneva, Switzerland.
7National Center of Competence in Research Chemical Biology, University of Geneva, CH-1211 Geneva, Switzerland.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Bastien Chopard
2department of Computer Science, University of Geneva, CH-1211 Geneva, Switzerland.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Aurélien Roux
1department of Biochemistry, University of Geneva, CH-1211 Geneva, Switzerland.
7National Center of Competence in Research Chemical Biology, University of Geneva, CH-1211 Geneva, Switzerland.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Aurélien Roux
  • For correspondence: Aurelien.Roux@unige.ch
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

Many organs, such as the gut or the spine are formed through folding of an epithelium. Whereas genetic regulation of epithelium folding has been investigated extensively, the nature of the mechanical forces driving this process remain largely unknown. Here we show that monolayers of identical cells proliferating on the inner surface of elastic spherical shells can spontaneously fold. By measuring the elastic deformation of the shell we inferred the forces acting within the monolayer. Using analytical and numerical theories at different scales, we found that the compressive stresses arising within the cell monolayer through proliferation quantitatively account for the shape of folds observed in experiments. Our study shows that forces arising from epithelium growth are sufficient to drive folding by buckling.

One Sentence Summary Epithelial cells proliferating in an artificial, elastic hollow sphere accumulate compressive stresses that drive inward buckling.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted January 07, 2019.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Buckling of epithelium growing under spherical confinement
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Buckling of epithelium growing under spherical confinement
Anastasiya Trushko, Ilaria Di Meglio, Aziza Merzouki, Carles Blanch-Mercader, Shada Abuhattum, Jochen Guck, Kevin Alessandri, Pierre Nassoy, Karsten Kruse, Bastien Chopard, Aurélien Roux
bioRxiv 513119; doi: https://doi.org/10.1101/513119
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
Buckling of epithelium growing under spherical confinement
Anastasiya Trushko, Ilaria Di Meglio, Aziza Merzouki, Carles Blanch-Mercader, Shada Abuhattum, Jochen Guck, Kevin Alessandri, Pierre Nassoy, Karsten Kruse, Bastien Chopard, Aurélien Roux
bioRxiv 513119; doi: https://doi.org/10.1101/513119

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Biophysics
Subject Areas
All Articles
  • Animal Behavior and Cognition (4095)
  • Biochemistry (8793)
  • Bioengineering (6495)
  • Bioinformatics (23406)
  • Biophysics (11769)
  • Cancer Biology (9173)
  • Cell Biology (13304)
  • Clinical Trials (138)
  • Developmental Biology (7426)
  • Ecology (11392)
  • Epidemiology (2066)
  • Evolutionary Biology (15127)
  • Genetics (10419)
  • Genomics (14029)
  • Immunology (9154)
  • Microbiology (22132)
  • Molecular Biology (8797)
  • Neuroscience (47470)
  • Paleontology (350)
  • Pathology (1423)
  • Pharmacology and Toxicology (2486)
  • Physiology (3712)
  • Plant Biology (8073)
  • Scientific Communication and Education (1434)
  • Synthetic Biology (2217)
  • Systems Biology (6023)
  • Zoology (1251)