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All-optical interrogation of a direction selective retinal circuit by holographic wave front shaping

G.L.B Spampinato, E. Ronzitti, V. Zampini, U. Ferrari, F. Trapani, H. Khabou, D. Dalkara, S. Picaud, E. Papagiakoumou, O. Marre, V. Emiliani
doi: https://doi.org/10.1101/513192
G.L.B Spampinato
1Institut de la Vision, Sorbonne Université, Inserm S968, CNRS UMR7210, 17 Rue Moreau, 75011
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E. Ronzitti
1Institut de la Vision, Sorbonne Université, Inserm S968, CNRS UMR7210, 17 Rue Moreau, 75011
2Wavefront-Engineering Microscopy group, Neurophotonics Laboratory, CNRS UMR8250, Paris Descartes University, 45 rue des Saints-Pères, Paris, France
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V. Zampini
1Institut de la Vision, Sorbonne Université, Inserm S968, CNRS UMR7210, 17 Rue Moreau, 75011
2Wavefront-Engineering Microscopy group, Neurophotonics Laboratory, CNRS UMR8250, Paris Descartes University, 45 rue des Saints-Pères, Paris, France
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U. Ferrari
1Institut de la Vision, Sorbonne Université, Inserm S968, CNRS UMR7210, 17 Rue Moreau, 75011
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F. Trapani
1Institut de la Vision, Sorbonne Université, Inserm S968, CNRS UMR7210, 17 Rue Moreau, 75011
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H. Khabou
1Institut de la Vision, Sorbonne Université, Inserm S968, CNRS UMR7210, 17 Rue Moreau, 75011
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D. Dalkara
1Institut de la Vision, Sorbonne Université, Inserm S968, CNRS UMR7210, 17 Rue Moreau, 75011
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S. Picaud
1Institut de la Vision, Sorbonne Université, Inserm S968, CNRS UMR7210, 17 Rue Moreau, 75011
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E. Papagiakoumou
1Institut de la Vision, Sorbonne Université, Inserm S968, CNRS UMR7210, 17 Rue Moreau, 75011
2Wavefront-Engineering Microscopy group, Neurophotonics Laboratory, CNRS UMR8250, Paris Descartes University, 45 rue des Saints-Pères, Paris, France
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O. Marre
1Institut de la Vision, Sorbonne Université, Inserm S968, CNRS UMR7210, 17 Rue Moreau, 75011
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V. Emiliani
1Institut de la Vision, Sorbonne Université, Inserm S968, CNRS UMR7210, 17 Rue Moreau, 75011
2Wavefront-Engineering Microscopy group, Neurophotonics Laboratory, CNRS UMR8250, Paris Descartes University, 45 rue des Saints-Pères, Paris, France
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  • For correspondence: valentina.emiliani@parisdescartes.fr
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Abstract

Direction selective (DS) ganglion cells (GC) in the retina maintain their tuning across a broad range of light levels. Yet very different circuits can shape their responses from bright to dim light, and their respective contributions are difficult to tease apart. In particular, the contribution of the rod bipolar cell (RBC) primary pathway, a key player in dim light, is unclear. To understand its contribution to DSGC response, we designed an all-optical approach allowing precise manipulation of single retinal neurons. Our system activates single cells in the bipolar cell (BC) layer by two-photon (2P) temporally focused holographic illumination, while recording the activity in the ganglion cell layer by 2P Ca2 imaging. By doing so, we demonstrate that RBCs provide an asymmetric input to DSGCs, suggesting they contribute to their direction selectivity. Our results suggest that every circuit providing an input to direction selective cells can generate direction selectivity by itself. This hints at a general principle to achieve robust selectivity in sensory areas.

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Posted January 06, 2019.
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All-optical interrogation of a direction selective retinal circuit by holographic wave front shaping
G.L.B Spampinato, E. Ronzitti, V. Zampini, U. Ferrari, F. Trapani, H. Khabou, D. Dalkara, S. Picaud, E. Papagiakoumou, O. Marre, V. Emiliani
bioRxiv 513192; doi: https://doi.org/10.1101/513192
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All-optical interrogation of a direction selective retinal circuit by holographic wave front shaping
G.L.B Spampinato, E. Ronzitti, V. Zampini, U. Ferrari, F. Trapani, H. Khabou, D. Dalkara, S. Picaud, E. Papagiakoumou, O. Marre, V. Emiliani
bioRxiv 513192; doi: https://doi.org/10.1101/513192

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