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A rabbit monoclonal antibody against the antiviral and cancer genomic DNA mutating enzyme APOBEC3B
William L. Brown, Emily K. Law, Michael A. Carpenter, Prokopios P. Argyris, Rena Levin-Klein, Alison N. Ranum, Amy M. Molan, Colleen L. Forster, Brett D. Anderson, Lela Lackey, Reuben S. Harris
doi: https://doi.org/10.1101/513341
William L. Brown
1Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, Minnesota, USA, 55455
2Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota, USA, 55455
3Institute for Molecular Virology, University of Minnesota, Minneapolis, Minnesota, USA, 55455
4Center for Genome Engineering, University of Minnesota, Minneapolis, Minnesota, USA, 55455
Emily K. Law
1Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, Minnesota, USA, 55455
2Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota, USA, 55455
3Institute for Molecular Virology, University of Minnesota, Minneapolis, Minnesota, USA, 55455
4Center for Genome Engineering, University of Minnesota, Minneapolis, Minnesota, USA, 55455
5Howard Hughes Medical Institute, University of Minnesota, Minneapolis, Minnesota, USA, 55455
Michael A. Carpenter
1Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, Minnesota, USA, 55455
2Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota, USA, 55455
3Institute for Molecular Virology, University of Minnesota, Minneapolis, Minnesota, USA, 55455
4Center for Genome Engineering, University of Minnesota, Minneapolis, Minnesota, USA, 55455
5Howard Hughes Medical Institute, University of Minnesota, Minneapolis, Minnesota, USA, 55455
Prokopios P. Argyris
1Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, Minnesota, USA, 55455
2Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota, USA, 55455
3Institute for Molecular Virology, University of Minnesota, Minneapolis, Minnesota, USA, 55455
4Center for Genome Engineering, University of Minnesota, Minneapolis, Minnesota, USA, 55455
6Division of Oral and Maxillofacial Pathology, School of Dentistry, University of Minnesota, Minneapolis, Minnesota, USA, 55455
Rena Levin-Klein
1Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, Minnesota, USA, 55455
2Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota, USA, 55455
3Institute for Molecular Virology, University of Minnesota, Minneapolis, Minnesota, USA, 55455
4Center for Genome Engineering, University of Minnesota, Minneapolis, Minnesota, USA, 55455
Alison N. Ranum
1Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, Minnesota, USA, 55455
2Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota, USA, 55455
3Institute for Molecular Virology, University of Minnesota, Minneapolis, Minnesota, USA, 55455
4Center for Genome Engineering, University of Minnesota, Minneapolis, Minnesota, USA, 55455
Amy M. Molan
1Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, Minnesota, USA, 55455
2Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota, USA, 55455
3Institute for Molecular Virology, University of Minnesota, Minneapolis, Minnesota, USA, 55455
4Center for Genome Engineering, University of Minnesota, Minneapolis, Minnesota, USA, 55455
Colleen L. Forster
7Clinical and Translational Science Institute, University of Minnesota, Minneapolis, Minnesota, USA, 55455
Brett D. Anderson
1Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, Minnesota, USA, 55455
2Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota, USA, 55455
3Institute for Molecular Virology, University of Minnesota, Minneapolis, Minnesota, USA, 55455
4Center for Genome Engineering, University of Minnesota, Minneapolis, Minnesota, USA, 55455
Lela Lackey
1Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, Minnesota, USA, 55455
2Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota, USA, 55455
3Institute for Molecular Virology, University of Minnesota, Minneapolis, Minnesota, USA, 55455
4Center for Genome Engineering, University of Minnesota, Minneapolis, Minnesota, USA, 55455
8Department of Biology, University of North Carolina, Chapel Hill, North Carolina, USA, 27599
Reuben S. Harris
1Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, Minnesota, USA, 55455
2Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota, USA, 55455
3Institute for Molecular Virology, University of Minnesota, Minneapolis, Minnesota, USA, 55455
4Center for Genome Engineering, University of Minnesota, Minneapolis, Minnesota, USA, 55455
5Howard Hughes Medical Institute, University of Minnesota, Minneapolis, Minnesota, USA, 55455
Abstract
The DNA cytosine deaminase APOBEC3B (A3B) is normally an antiviral factor in the innate immune response. However, A3B has been implicated in cancer mutagenesis, particularly in solid tumors of the bladder, breast, cervix, head/neck, and lung. Here, we report data on the generation and characterization of a rabbit monoclonal antibody (mAb) for human A3B through a series of immunoblot, immunofluorescence microscopy, and immunohistochemistry experiments. Positive results indicate that one mAb, 5210-87-13, will be useful for fundamental research in virology and cancer biology and that immunohistochemical quantification of A3B enzyme levels may constitute a clinical biomarker for tumor evolvability and differential treatment.
Copyright
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Posted January 07, 2019.
A rabbit monoclonal antibody against the antiviral and cancer genomic DNA mutating enzyme APOBEC3B
William L. Brown, Emily K. Law, Michael A. Carpenter, Prokopios P. Argyris, Rena Levin-Klein, Alison N. Ranum, Amy M. Molan, Colleen L. Forster, Brett D. Anderson, Lela Lackey, Reuben S. Harris
bioRxiv 513341; doi: https://doi.org/10.1101/513341
A rabbit monoclonal antibody against the antiviral and cancer genomic DNA mutating enzyme APOBEC3B
William L. Brown, Emily K. Law, Michael A. Carpenter, Prokopios P. Argyris, Rena Levin-Klein, Alison N. Ranum, Amy M. Molan, Colleen L. Forster, Brett D. Anderson, Lela Lackey, Reuben S. Harris
bioRxiv 513341; doi: https://doi.org/10.1101/513341
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