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A genetic system for biasing the sex ratio in mice

Ido Yosef, Liat Edry-Botzer, Rea Globus, Inbar Shlomovitz, Ariel Munitz, Motti Gerlic, Udi Qimron
doi: https://doi.org/10.1101/515064
Ido Yosef
Department of Clinical Microbiology and Immunology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel
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Liat Edry-Botzer
Department of Clinical Microbiology and Immunology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel
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Rea Globus
Department of Clinical Microbiology and Immunology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel
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Inbar Shlomovitz
Department of Clinical Microbiology and Immunology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel
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Ariel Munitz
Department of Clinical Microbiology and Immunology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel
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Motti Gerlic
Department of Clinical Microbiology and Immunology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel
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  • For correspondence: mgerlic@post.tau.ac.il ehudq@post.tau.ac.il
Udi Qimron
Department of Clinical Microbiology and Immunology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel
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  • For correspondence: mgerlic@post.tau.ac.il ehudq@post.tau.ac.il
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Abstract

The ability to preselect the sex of livestock is economically beneficial and significantly increases the welfare and proper use of animals. In the poultry industry, for example, almost all males are brutally and unnecessarily killed shortly after hatching. The labor and associated costs of separation of females from males, as well as the massive killing of males, could be reduced by using a system that biases the sex of the progeny. Here, we provide a first proof of concept for such a system by crossing two genetically engineered mouse lines. The maternal line encodes a functional Cas9 protein on an autosomal chromosome, whereas the paternal line encodes guide RNAs on the Y chromosome targeting vital mouse genes. After fertilization, the presence of both the Y-encoded guide RNAs from the paternal sperm and the Cas9 protein from the maternal egg target the vital genes in males. We show that this breeding consequently self-destructs solely the males. Our results pave the way for a biased sex production of livestock, thus saving labor, costs, and eliminating substantial animal suffering.

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Posted January 20, 2019.
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A genetic system for biasing the sex ratio in mice
Ido Yosef, Liat Edry-Botzer, Rea Globus, Inbar Shlomovitz, Ariel Munitz, Motti Gerlic, Udi Qimron
bioRxiv 515064; doi: https://doi.org/10.1101/515064
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A genetic system for biasing the sex ratio in mice
Ido Yosef, Liat Edry-Botzer, Rea Globus, Inbar Shlomovitz, Ariel Munitz, Motti Gerlic, Udi Qimron
bioRxiv 515064; doi: https://doi.org/10.1101/515064

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