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Follicle-stimulating hormone and luteinizing hormone increase Ca2+ in the granulosa cells of mouse ovarian follicles1

Jeremy R. Egbert, Paul G. Fahey, Jacob Reimer, Corie M. Owen, Alexei V. Evsikov, Viacheslav O. Nikolaev, Oliver Griesbeck, Russell S. Ray, Andreas S. Tolias, Laurinda A. Jaffe
doi: https://doi.org/10.1101/520122
Jeremy R. Egbert
Department of Cell Biology, UConn Health, Farmington, CT, USA
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  • For correspondence: egbert@uchc.edu
Paul G. Fahey
Department of Neuroscience, Baylor College of Medicine, Houston, TX, USA
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Jacob Reimer
Department of Neuroscience, Baylor College of Medicine, Houston, TX, USA
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Corie M. Owen
Department of Cell Biology, UConn Health, Farmington, CT, USA
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Alexei V. Evsikov
Department of Research and Development, Bay Pines Veteran Administration Healthcare System, Bay Pines, FL, USA
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Viacheslav O. Nikolaev
Institute of Experimental Cardiovascular Research, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
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Oliver Griesbeck
Max Planck Institute of Neurobiology, Martinsried, Germany
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Russell S. Ray
Department of Neuroscience, Baylor College of Medicine, Houston, TX, USA
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Andreas S. Tolias
Department of Neuroscience, Baylor College of Medicine, Houston, TX, USACenter for Neuroscience and Artificial Intelligence, Baylor College of Medicine, Houston, TX, USA
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Laurinda A. Jaffe
Department of Cell Biology, UConn Health, Farmington, CT, USA
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Abstract

Summary sentence Both FSH and LH increase Ca2+ in the granulosa cells of intact ovarian follicles from mice expressing genetically encoded sensors.

ABSTRACT In mammalian ovarian follicles, follicle stimulating hormone (FSH) and luteinizing hormone (LH) signal primarily through the G-protein Gs to elevate cAMP, but both of these hormones can also elevate Ca2+ under some conditions. Here we investigate FSH- and LH-induced Ca2+ signaling in intact follicles of mice expressing genetically encoded Ca2+ sensors, Twitch-2B and GCaMP6s. At a physiological concentration (1 nM), FSH elevates Ca2+ within the granulosa cells of preantral and antral follicles. The Ca2+ rise begins several minutes after FSH application, peaks at ~10 minutes, remains above baseline for another ~10 minutes, and depends on extracellular Ca2+. However, suppression of the FSH-induced Ca2+ increase by reducing extracellular Ca2+ does not inhibit FSH-induced phosphorylation of MAP kinase, estradiol production, or the acquisition of LH responsiveness. Like FSH, LH also increases Ca2+, when applied to preovulatory follicles. At a physiological concentration (10 nM), LH elicits Ca2+ oscillations in a subset of cells in the outer mural granulosa layer. These oscillations continue for at least 6 hours and depend on the activity of Gq family G-proteins. Suppression of the oscillations by Gq inhibition does not inhibit meiotic resumption, but does slightly attenuate ovulation. In summary, both FSH and LH increase Ca2+ in the granulosa cells of intact follicles, but the functions of these Ca2+ rises are only starting to be identified.

Footnotes

  • 1 Supported by NIH grant R37HD014939 to L.A.J., by grants from the Fund for Science to J.R.E. and to A.V.E., and by the Intelligence Advanced Research Projects Activity (IARPA) via Department of Interior/Interior Business Center (DoI/IBC) contract number D16PC00003 to A.S.T. The U.S. Government is authorized to reproduce and distribute reprints for Governmental purposes notwithstanding any copyright annotation thereon. Disclaimer: The views and conclusions contained herein are those of the authors and should not be interpreted as necessarily representing the official policies or endorsements, either expressed or implied, of IARPA, DoI/IBC, or the U.S. Government. Presented in part at the 51st Annual Meeting of the Society for the Study of Reproduction, 10-13 July, 2018, New Orleans, LA, USA.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted January 14, 2019.
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Follicle-stimulating hormone and luteinizing hormone increase Ca2+ in the granulosa cells of mouse ovarian follicles1
Jeremy R. Egbert, Paul G. Fahey, Jacob Reimer, Corie M. Owen, Alexei V. Evsikov, Viacheslav O. Nikolaev, Oliver Griesbeck, Russell S. Ray, Andreas S. Tolias, Laurinda A. Jaffe
bioRxiv 520122; doi: https://doi.org/10.1101/520122
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Follicle-stimulating hormone and luteinizing hormone increase Ca2+ in the granulosa cells of mouse ovarian follicles1
Jeremy R. Egbert, Paul G. Fahey, Jacob Reimer, Corie M. Owen, Alexei V. Evsikov, Viacheslav O. Nikolaev, Oliver Griesbeck, Russell S. Ray, Andreas S. Tolias, Laurinda A. Jaffe
bioRxiv 520122; doi: https://doi.org/10.1101/520122

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