Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Exercise-stimulated muscle ROS production and glucose uptake requires NADPH oxidase 2

View ORCID ProfileCarlos Henriquez-Olguin, Jonas R. Knudsen, Steffen H. Raun, Zhencheng Li, Emilie Dalbram, Jonas T. Treebak, Lykke Sylow, Rikard Holmdahl, Erik A. Richter, Enrique Jaimovich, View ORCID ProfileThomas E. Jensen
doi: https://doi.org/10.1101/522805
Carlos Henriquez-Olguin
University of Copenhagen;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Carlos Henriquez-Olguin
Jonas R. Knudsen
Department of Nutrition, Exercise and Sports, University of Copenhagen.;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Steffen H. Raun
Department of Nutrition, Exercise and Sports, University of Copenhagen.;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Zhencheng Li
Department of Nutrition, Exercise and Sports, University of Copenhagen.;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Emilie Dalbram
Novo Nordisk Foundation Center for Basic Metabolic Research, University of Copenhagen.;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jonas T. Treebak
Novo Nordisk Foundation Center for Basic Metabolic Research, University of Copenhagen.;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Lykke Sylow
Department of Nutrition, Exercise and Sports, University of Copenhagen.;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Rikard Holmdahl
Department of Medical Biochemistry and Biophysics, Karolinska Institute.;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Erik A. Richter
Department of Nutrition, Exercise and Sports, University of Copenhagen.;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Enrique Jaimovich
Center of Exercise, Metabolism and Cancer, Biomedical Sciences Institute, Universidad de Chile.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Thomas E. Jensen
Department of Nutrition, Exercise and Sports, University of Copenhagen.;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Thomas E. Jensen
  • For correspondence: tejensen@nexs.ku.dk
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

Reactive oxygen species (ROS) act as intracellular compartmentalized second messengers mediating metabolic stress-adaptation. In skeletal muscle fibers, ROS have been suggested to stimulate glucose transporter 4 (GLUT4)-dependent glucose transport during artificially evoked contraction ex vivo but whether myocellular ROS production is stimulated by in vivo exercise to control metabolism is unclear. Here, we combined exercise in humans and mice with fluorescent dyes, genetically-encoded biosensors, and NADPH oxidase 2 (NOX2) loss-of-function models to demonstrate that NOX2 is the main source of ROS during moderate-intensity exercise in skeletal muscle. Furthermore, two NOX2 loss-of-function mouse models lacking either p47phox or Rac1 presented striking phenotypic similarities, including greatly reduced exercise-stimulated glucose uptake and GLUT4 translocation. These findings indicate that NOX2 is a major myocellular ROS source regulating glucose transport capacity during moderate intensity exercise.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-ND 4.0 International license.
Back to top
PreviousNext
Posted January 17, 2019.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Exercise-stimulated muscle ROS production and glucose uptake requires NADPH oxidase 2
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
Share
Exercise-stimulated muscle ROS production and glucose uptake requires NADPH oxidase 2
Carlos Henriquez-Olguin, Jonas R. Knudsen, Steffen H. Raun, Zhencheng Li, Emilie Dalbram, Jonas T. Treebak, Lykke Sylow, Rikard Holmdahl, Erik A. Richter, Enrique Jaimovich, Thomas E. Jensen
bioRxiv 522805; doi: https://doi.org/10.1101/522805
Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
Exercise-stimulated muscle ROS production and glucose uptake requires NADPH oxidase 2
Carlos Henriquez-Olguin, Jonas R. Knudsen, Steffen H. Raun, Zhencheng Li, Emilie Dalbram, Jonas T. Treebak, Lykke Sylow, Rikard Holmdahl, Erik A. Richter, Enrique Jaimovich, Thomas E. Jensen
bioRxiv 522805; doi: https://doi.org/10.1101/522805

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Physiology
Subject Areas
All Articles
  • Animal Behavior and Cognition (996)
  • Biochemistry (1485)
  • Bioengineering (938)
  • Bioinformatics (6803)
  • Biophysics (2414)
  • Cancer Biology (1782)
  • Cell Biology (2514)
  • Clinical Trials (106)
  • Developmental Biology (1683)
  • Ecology (2553)
  • Epidemiology (1488)
  • Evolutionary Biology (5003)
  • Genetics (3598)
  • Genomics (4614)
  • Immunology (1156)
  • Microbiology (4222)
  • Molecular Biology (1617)
  • Neuroscience (10740)
  • Paleontology (81)
  • Pathology (236)
  • Pharmacology and Toxicology (407)
  • Physiology (552)
  • Plant Biology (1443)
  • Scientific Communication and Education (410)
  • Synthetic Biology (542)
  • Systems Biology (1868)
  • Zoology (257)