Accurate detection of m6A RNA modifications in native RNA sequences

Huanle Liu; Oguzhan Begik; Morghan C Lucas; Christopher E. Mason; Schraga Schwartz; John S. Mattick; Martin A. Smith; Eva Maria Novoa

doi:10.1101/525741

ABSTRACT

The field of epitranscriptomics has undergone an enormous expansion in the last few years; however, a major limitation is the lack of generic methods to map RNA modifications transcriptome-wide. Here we show that using Oxford Nanopore Technologies, N6-methyladenosine (m6A) RNA modifications can be detected with high accuracy, in the form of systematic errors and decreased base-calling qualities. Our results open new avenues to investigate the universe of RNA modifications with single nucleotide resolution, in individual RNA molecules.

LIST OF ABBREVIATIONS

(m6A): N6-methlyadenosine
(m1A): N1-methyladenosine
(5hmc): 5-hydroxymethylcytosine
(ONT): Oxford Nanopore Technologies
(SVM): Support Vector Machine
(IVT): In vitro transcription

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