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Cell type composition and circuit organization of neocortical radial clones

View ORCID ProfileCathryn R. Cadwell, Federico Scala, Paul G. Fahey, View ORCID ProfileDmitry Kobak, View ORCID ProfileFabian H. Sinz, Per Johnsson, Shuang Li, R. James Cotton, View ORCID ProfileRickard Sandberg, View ORCID ProfilePhilipp Berens, View ORCID ProfileXiaolong Jiang, Andreas S. Tolias
doi: https://doi.org/10.1101/526681
Cathryn R. Cadwell
1Department of Neuroscience, Baylor College of Medicine, Houston, TX, USA
2Center for Neuroscience and Artificial Intelligence, Baylor College of Medicine, Houston, TX, USA
3Department of Anatomic Pathology, University of California San Francisco, San Francisco, CA, USA
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Federico Scala
1Department of Neuroscience, Baylor College of Medicine, Houston, TX, USA
2Center for Neuroscience and Artificial Intelligence, Baylor College of Medicine, Houston, TX, USA
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Paul G. Fahey
1Department of Neuroscience, Baylor College of Medicine, Houston, TX, USA
2Center for Neuroscience and Artificial Intelligence, Baylor College of Medicine, Houston, TX, USA
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Dmitry Kobak
4Institute for Ophthalmic Research, University of Tübingen, Tübingen, Germany
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Fabian H. Sinz
1Department of Neuroscience, Baylor College of Medicine, Houston, TX, USA
2Center for Neuroscience and Artificial Intelligence, Baylor College of Medicine, Houston, TX, USA
5Department of Computer Science, University of Tübingen, Tübingen, Germany
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Per Johnsson
6Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden
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Shuang Li
1Department of Neuroscience, Baylor College of Medicine, Houston, TX, USA
2Center for Neuroscience and Artificial Intelligence, Baylor College of Medicine, Houston, TX, USA
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R. James Cotton
1Department of Neuroscience, Baylor College of Medicine, Houston, TX, USA
2Center for Neuroscience and Artificial Intelligence, Baylor College of Medicine, Houston, TX, USA
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Rickard Sandberg
6Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden
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Philipp Berens
4Institute for Ophthalmic Research, University of Tübingen, Tübingen, Germany
5Department of Computer Science, University of Tübingen, Tübingen, Germany
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Xiaolong Jiang
1Department of Neuroscience, Baylor College of Medicine, Houston, TX, USA
2Center for Neuroscience and Artificial Intelligence, Baylor College of Medicine, Houston, TX, USA
7Jan and Dan Duncan Neurological Research Institute at Texas Children’s Hospital, Houston, Texas, USA
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Andreas S. Tolias
1Department of Neuroscience, Baylor College of Medicine, Houston, TX, USA
2Center for Neuroscience and Artificial Intelligence, Baylor College of Medicine, Houston, TX, USA
8Department of Electrical and Computer Engineering, Rice University, Houston, Texas, USA
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Abstract

Summary Excitatory neurons arising from a common progenitor establish radially-oriented clonal units in the neocortex which have been proposed to serve as elementary information processing modules. To characterize the cell types and circuit diagram within these clonal units, we performed single-cell RNA-sequencing and multi-cell patch clamp recordings of neurons derived from Nestin-positive progenitors. We found that radial clones do not appear to be fate-restricted, but instead individual clones are composed of a random sampling of the transcriptomic cell types present in a particular cortical area. The effect of lineage on synaptic connectivity depends on the type of connection tested: pairs of clonally related neurons were more likely to be connected vertically, across cortical layers, but not laterally within the same layer, compared to unrelated pairs. We propose that integration of vertical input from related neurons with lateral input from unrelated neurons may represent a developmentally programmed motif for assembling neocortical circuits.

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Posted January 23, 2019.
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Cell type composition and circuit organization of neocortical radial clones
Cathryn R. Cadwell, Federico Scala, Paul G. Fahey, Dmitry Kobak, Fabian H. Sinz, Per Johnsson, Shuang Li, R. James Cotton, Rickard Sandberg, Philipp Berens, Xiaolong Jiang, Andreas S. Tolias
bioRxiv 526681; doi: https://doi.org/10.1101/526681
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Cell type composition and circuit organization of neocortical radial clones
Cathryn R. Cadwell, Federico Scala, Paul G. Fahey, Dmitry Kobak, Fabian H. Sinz, Per Johnsson, Shuang Li, R. James Cotton, Rickard Sandberg, Philipp Berens, Xiaolong Jiang, Andreas S. Tolias
bioRxiv 526681; doi: https://doi.org/10.1101/526681

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