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Mycobacterium abscessus virulence traits unraveled by transcriptomic profiling in amoeba and macrophages

Violaine Dubois, Alexandre Pawlik, Anouchka Bories, Vincent Le Moigne, Odile Sismeiro, Rachel Legendre, Hugo Varet, Pilar Rodriguez, Jean-Louis Gaillard, Jean-Yves Copee, Roland Brosch, Jean-Louis Herrmann, View ORCID ProfileFabienne Girard-misguich
doi: https://doi.org/10.1101/529057
Violaine Dubois
Universite Paris-Saclay;
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Alexandre Pawlik
Institut Pasteur
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Anouchka Bories
Universite Paris-Saclay;
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Vincent Le Moigne
Universite Paris-Saclay;
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Odile Sismeiro
Institut Pasteur
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Rachel Legendre
Institut Pasteur
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Hugo Varet
Institut Pasteur
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Pilar Rodriguez
Universite Paris-Saclay;
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Jean-Louis Gaillard
Universite Paris-Saclay;
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Jean-Yves Copee
Institut Pasteur
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Roland Brosch
Institut Pasteur
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Jean-Louis Herrmann
Universite Paris-Saclay;
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Fabienne Girard-misguich
Universite Paris-Saclay;
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  • ORCID record for Fabienne Girard-misguich
  • For correspondence: fabienne.misguich@uvsq.fr
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Abstract

Free-living amoebae might represent an evolutionary niche. In order to get more insights into the potential amoebal training ground for Mycobacterium abscessus, we characterized its full transcriptome in amoeba (Ac) and macrophages (MÞ), as well as the Mycobacterium chelonae intra-Ac transcriptome for comparison. Up-regulated genes in Ac allowed M. abscessus to resist environmental stress and induce defense mechanisms, as well as showing switch from carbohydrate carbon sources to fatty acid metabolism. Eleven genes implicated in the adaptation to intracellular stress, were mutated, with all but one confirmed to be involved in M. abscessus intra-MÞ survival. Cloning two of these genes in M. chelonae increased its intra-MÞ survival. One mutant was particularly attenuated in MÞ that corresponded to the deletion of an Eis N-acetyl transferase protein (MAB_4532c). Taken together, M. abscessus transcriptomes revealed the intracellular lifestyle of the mycobacteria, with Ac largely contributing to the enhancement of M. abscessus intra-MÞ survival.

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Posted January 23, 2019.
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Mycobacterium abscessus virulence traits unraveled by transcriptomic profiling in amoeba and macrophages
Violaine Dubois, Alexandre Pawlik, Anouchka Bories, Vincent Le Moigne, Odile Sismeiro, Rachel Legendre, Hugo Varet, Pilar Rodriguez, Jean-Louis Gaillard, Jean-Yves Copee, Roland Brosch, Jean-Louis Herrmann, Fabienne Girard-misguich
bioRxiv 529057; doi: https://doi.org/10.1101/529057
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Mycobacterium abscessus virulence traits unraveled by transcriptomic profiling in amoeba and macrophages
Violaine Dubois, Alexandre Pawlik, Anouchka Bories, Vincent Le Moigne, Odile Sismeiro, Rachel Legendre, Hugo Varet, Pilar Rodriguez, Jean-Louis Gaillard, Jean-Yves Copee, Roland Brosch, Jean-Louis Herrmann, Fabienne Girard-misguich
bioRxiv 529057; doi: https://doi.org/10.1101/529057

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