Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Physicochemical Properties of Chromosomes in Live Cells Characterized by Label-Free Imaging and Fluorescence Correlation Spectroscopy

Tae-Keun Kim, Byong-Wook Lee, Fumihiko Fujii, Kee-Hang Lee, YongKeun Park, Jun Ki Kim, Chan-Gi Pack
doi: https://doi.org/10.1101/533596
Tae-Keun Kim
1Asan Institute for Life Sciences, Asan Medical Center, Seoul 05505, Republic of Korea
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Byong-Wook Lee
1Asan Institute for Life Sciences, Asan Medical Center, Seoul 05505, Republic of Korea
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Fumihiko Fujii
2Division of Physical Pharmacy, Faculty of Pharmaceutical Sciences, Kobe Gakuin University, Kobe 650-8586, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kee-Hang Lee
3TOMOCUBE Inc., Daejeon 34051, Republic of Korea
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
YongKeun Park
4Department of Physics, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jun Ki Kim
1Asan Institute for Life Sciences, Asan Medical Center, Seoul 05505, Republic of Korea
5Department of Convergence Medicine, University of Ulsan College of Medicine, Seoul 05505, Republic of Korea
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: kim@amc.seoul.kr changipack@amc.seoul.kr
Chan-Gi Pack
1Asan Institute for Life Sciences, Asan Medical Center, Seoul 05505, Republic of Korea
5Department of Convergence Medicine, University of Ulsan College of Medicine, Seoul 05505, Republic of Korea
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: kim@amc.seoul.kr changipack@amc.seoul.kr
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

The cell nucleus is a three-dimensional, dynamic organelle that is organized into many subnuclear bodies, such as chromatin and nucleoli. The structure and function of these bodies is maintained by diffusion and interactions between related factors as well as dynamic and structural changes. Recent studies using fluorescent microscopic techniques suggest that protein factors can access and are freely mobile in mitotic chromosomes, despite their densely packed structure. However, the physicochemical properties of the chromosome itself during cell division are not yet fully understood. Physical parameters, such as the refractive index (RI), volume of the mitotic chromosome, and diffusion coefficients of fluorescent probes inside the chromosome were quantified using an approach combining label-free optical diffraction tomography with complementary confocal laser scanning microscopy and fluorescence correlation spectroscopy. Variance in these parameters correlated among various osmotic conditions, suggesting that changes in RI are consistent with those in the diffusion coefficient for mitotic chromosomes and cytosol. Serial RI tomography images of chromosomes in live cells during mitosis were compared with three-dimensional confocal micrographs to demonstrate that compaction and decompaction of chromosomes induced by osmotic change were characterized by linked changes in chromosome RI, volume, and the mobility of fluorescent proteins.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
Back to top
PreviousNext
Posted January 29, 2019.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Physicochemical Properties of Chromosomes in Live Cells Characterized by Label-Free Imaging and Fluorescence Correlation Spectroscopy
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Physicochemical Properties of Chromosomes in Live Cells Characterized by Label-Free Imaging and Fluorescence Correlation Spectroscopy
Tae-Keun Kim, Byong-Wook Lee, Fumihiko Fujii, Kee-Hang Lee, YongKeun Park, Jun Ki Kim, Chan-Gi Pack
bioRxiv 533596; doi: https://doi.org/10.1101/533596
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
Physicochemical Properties of Chromosomes in Live Cells Characterized by Label-Free Imaging and Fluorescence Correlation Spectroscopy
Tae-Keun Kim, Byong-Wook Lee, Fumihiko Fujii, Kee-Hang Lee, YongKeun Park, Jun Ki Kim, Chan-Gi Pack
bioRxiv 533596; doi: https://doi.org/10.1101/533596

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Biophysics
Subject Areas
All Articles
  • Animal Behavior and Cognition (3590)
  • Biochemistry (7562)
  • Bioengineering (5503)
  • Bioinformatics (20753)
  • Biophysics (10308)
  • Cancer Biology (7964)
  • Cell Biology (11625)
  • Clinical Trials (138)
  • Developmental Biology (6598)
  • Ecology (10177)
  • Epidemiology (2065)
  • Evolutionary Biology (13589)
  • Genetics (9530)
  • Genomics (12830)
  • Immunology (7917)
  • Microbiology (19525)
  • Molecular Biology (7651)
  • Neuroscience (42025)
  • Paleontology (307)
  • Pathology (1254)
  • Pharmacology and Toxicology (2195)
  • Physiology (3261)
  • Plant Biology (7028)
  • Scientific Communication and Education (1294)
  • Synthetic Biology (1949)
  • Systems Biology (5422)
  • Zoology (1113)