Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Loss of centromere function drives karyotype evolution in closely related Malassezia species

View ORCID ProfileSundar Ram Sankaranarayanan, Giuseppe Ianiri, Marco A. Coelho, Md. Hashim Reza, Bhagya C. Thimmappa, Promit Ganguly, Rakesh Netha Vadnala, Sheng Sun, Rahul Siddharthan, Christian Tellgren-Roth, Thomas L Dawson Jr., Joseph Heitman, View ORCID ProfileKaustuv Sanyal
doi: https://doi.org/10.1101/533794
Sundar Ram Sankaranarayanan
1Molecular Mycology Laboratory, Molecular Biology and Genetics Unit, Jawaharlal Nehru Center for Advanced Scientific Research, Jakkur P.O, Bengaluru-560064
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Sundar Ram Sankaranarayanan
Giuseppe Ianiri
2Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Marco A. Coelho
2Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Md. Hashim Reza
1Molecular Mycology Laboratory, Molecular Biology and Genetics Unit, Jawaharlal Nehru Center for Advanced Scientific Research, Jakkur P.O, Bengaluru-560064
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Bhagya C. Thimmappa
1Molecular Mycology Laboratory, Molecular Biology and Genetics Unit, Jawaharlal Nehru Center for Advanced Scientific Research, Jakkur P.O, Bengaluru-560064
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Promit Ganguly
1Molecular Mycology Laboratory, Molecular Biology and Genetics Unit, Jawaharlal Nehru Center for Advanced Scientific Research, Jakkur P.O, Bengaluru-560064
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Rakesh Netha Vadnala
3The Institute of Mathematical Sciences/HBNI, Taramani, Chennai 600113, India
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sheng Sun
2Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Rahul Siddharthan
3The Institute of Mathematical Sciences/HBNI, Taramani, Chennai 600113, India
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Christian Tellgren-Roth
4National Genomics Infrastructure, Science for Life Laboratory, Department of Immunology, Genetics and Pathology, Uppsala University, 75108 Uppsala, Sweden
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Thomas L Dawson Jr.
5Skin Research Institute Singapore, Agency for Science, Technology, and Research (A*STAR), 138648, Singapore
6Medical University of South Carolina, School of Pharmacy, Department of Drug Discovery, 29425, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Joseph Heitman
2Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: sanyal@jncasr.ac.in heitm001@duke.edu
Kaustuv Sanyal
1Molecular Mycology Laboratory, Molecular Biology and Genetics Unit, Jawaharlal Nehru Center for Advanced Scientific Research, Jakkur P.O, Bengaluru-560064
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Kaustuv Sanyal
  • For correspondence: sanyal@jncasr.ac.in heitm001@duke.edu
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

Genomic rearrangements associated with speciation often result in chromosome number variation among closely related species. Malassezia species show variable karyotypes ranging between 6 and 9 chromosomes. Here, we experimentally identified all 8 centromeres in M. sympodialis as 3 to 5 kb long kinetochore-bound regions spanning an AT-rich core and depleted of the canonical histone H3. Centromeres of similar sequence features were identified as CENP-A-rich regions in Malassezia furfur with 7 chromosomes, and histone H3 depleted regions in Malassezia slooffiae and Malassezia globosa with 9 chromosomes each. Analysis of synteny conservation across centromeres with newly generated chromosome-level genome assemblies suggests two distinct mechanisms of chromosome number reduction from an inferred 9-chromosome ancestral state: (a) chromosome breakage followed by loss of centromere DNA and (b) centromere inactivation accompanied by changes in DNA sequence following chromosome-chromosome fusion. We propose AT-rich centromeres drive karyotype diversity in the Malassezia species complex through breakage and inactivation.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted November 26, 2019.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Loss of centromere function drives karyotype evolution in closely related Malassezia species
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Loss of centromere function drives karyotype evolution in closely related Malassezia species
Sundar Ram Sankaranarayanan, Giuseppe Ianiri, Marco A. Coelho, Md. Hashim Reza, Bhagya C. Thimmappa, Promit Ganguly, Rakesh Netha Vadnala, Sheng Sun, Rahul Siddharthan, Christian Tellgren-Roth, Thomas L Dawson Jr., Joseph Heitman, Kaustuv Sanyal
bioRxiv 533794; doi: https://doi.org/10.1101/533794
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
Loss of centromere function drives karyotype evolution in closely related Malassezia species
Sundar Ram Sankaranarayanan, Giuseppe Ianiri, Marco A. Coelho, Md. Hashim Reza, Bhagya C. Thimmappa, Promit Ganguly, Rakesh Netha Vadnala, Sheng Sun, Rahul Siddharthan, Christian Tellgren-Roth, Thomas L Dawson Jr., Joseph Heitman, Kaustuv Sanyal
bioRxiv 533794; doi: https://doi.org/10.1101/533794

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Genomics
Subject Areas
All Articles
  • Animal Behavior and Cognition (4397)
  • Biochemistry (9632)
  • Bioengineering (7123)
  • Bioinformatics (24940)
  • Biophysics (12671)
  • Cancer Biology (9998)
  • Cell Biology (14405)
  • Clinical Trials (138)
  • Developmental Biology (7989)
  • Ecology (12148)
  • Epidemiology (2067)
  • Evolutionary Biology (16026)
  • Genetics (10953)
  • Genomics (14778)
  • Immunology (9907)
  • Microbiology (23739)
  • Molecular Biology (9508)
  • Neuroscience (51056)
  • Paleontology (370)
  • Pathology (1545)
  • Pharmacology and Toxicology (2694)
  • Physiology (4038)
  • Plant Biology (8696)
  • Scientific Communication and Education (1512)
  • Synthetic Biology (2404)
  • Systems Biology (6459)
  • Zoology (1350)