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Reconstruction of 1,000 projection neurons reveals new cell types and organization of long-range connectivity in the mouse brain

Johan Winnubst, Erhan Bas, Tiago A. Ferreira, Zhuhao Wu, Michael N. Economo, Patrick Edson, Ben J. Arthur, Christopher Bruns, Konrad Rokicki, David Schauder, Donald J. Olbris, Sean D. Murphy, David G. Ackerman, Cameron Arshadi, Perry Baldwin, Regina Blake, Ahmad Elsayed, Mashtura Hasan, Daniel Ramirez, Bruno Dos Santos, Monet Weldon, Amina Zafar, Joshua T. Dudmann, Charles R. Gerfen, Adam W. Hantman, Wyatt Korff, Scott M. Sternson, Nelson Spruston, Karel Svoboda, Jayaram Chandrashekar
doi: https://doi.org/10.1101/537233
Johan Winnubst
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
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Erhan Bas
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
2Amazon Web Services, Seattle, WA 98101, USA
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Tiago A. Ferreira
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
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Zhuhao Wu
3Laboratory of Molecular Genetics, The Rockefeller University, New York, NY 10065, USA
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Michael N. Economo
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
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Patrick Edson
4Leap Scientific LLC, Hooksett, NH 03106, USA
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Ben J. Arthur
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
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Christopher Bruns
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
5Environmental Systems Research Institute, Redlands, CA 92373, USA
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Konrad Rokicki
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
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David Schauder
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
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Donald J. Olbris
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
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Sean D. Murphy
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
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David G. Ackerman
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
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Cameron Arshadi
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
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Perry Baldwin
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
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Regina Blake
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
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Ahmad Elsayed
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
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Mashtura Hasan
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
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Daniel Ramirez
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
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Bruno Dos Santos
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
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Monet Weldon
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
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Amina Zafar
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
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Joshua T. Dudmann
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
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Charles R. Gerfen
6Intramural Research Program, National Institute of Mental Health, Bethesda, MD 20892, USA
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Adam W. Hantman
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
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Wyatt Korff
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
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Scott M. Sternson
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
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Nelson Spruston
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
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Karel Svoboda
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
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Jayaram Chandrashekar
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
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Summary

Neuronal cell types are the nodes of neural circuits that determine the flow of information within the brain. Neuronal morphology, especially the shape of the axonal arbor, provides an essential descriptor of cell type and reveals how individual neurons route their output across the brain. Despite the importance of morphology, few projection neurons in the mouse brain have been reconstructed in their entirety. Here we present a robust and efficient platform for imaging and reconstructing complete neuronal morphologies, including axonal arbors that span substantial portions of the brain. We used this platform to reconstruct more than 1,000 projection neurons in the motor cortex, thalamus, subiculum, and hypothalamus. Together, the reconstructed neurons comprise more than 75 meters of axonal length and are available in a searchable online database. Axonal shapes revealed previously unknown subtypes of projection neurons and suggest organizational principles of long-range connectivity.

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Posted February 01, 2019.
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Reconstruction of 1,000 projection neurons reveals new cell types and organization of long-range connectivity in the mouse brain
Johan Winnubst, Erhan Bas, Tiago A. Ferreira, Zhuhao Wu, Michael N. Economo, Patrick Edson, Ben J. Arthur, Christopher Bruns, Konrad Rokicki, David Schauder, Donald J. Olbris, Sean D. Murphy, David G. Ackerman, Cameron Arshadi, Perry Baldwin, Regina Blake, Ahmad Elsayed, Mashtura Hasan, Daniel Ramirez, Bruno Dos Santos, Monet Weldon, Amina Zafar, Joshua T. Dudmann, Charles R. Gerfen, Adam W. Hantman, Wyatt Korff, Scott M. Sternson, Nelson Spruston, Karel Svoboda, Jayaram Chandrashekar
bioRxiv 537233; doi: https://doi.org/10.1101/537233
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Reconstruction of 1,000 projection neurons reveals new cell types and organization of long-range connectivity in the mouse brain
Johan Winnubst, Erhan Bas, Tiago A. Ferreira, Zhuhao Wu, Michael N. Economo, Patrick Edson, Ben J. Arthur, Christopher Bruns, Konrad Rokicki, David Schauder, Donald J. Olbris, Sean D. Murphy, David G. Ackerman, Cameron Arshadi, Perry Baldwin, Regina Blake, Ahmad Elsayed, Mashtura Hasan, Daniel Ramirez, Bruno Dos Santos, Monet Weldon, Amina Zafar, Joshua T. Dudmann, Charles R. Gerfen, Adam W. Hantman, Wyatt Korff, Scott M. Sternson, Nelson Spruston, Karel Svoboda, Jayaram Chandrashekar
bioRxiv 537233; doi: https://doi.org/10.1101/537233

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