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Central spindle microtubules are strongly coupled to chromosomes during both anaphase A and anaphase B

Che-Hang Yu, Stefanie Redemann, Hai-Yin Wu, Robert Kiewisz, Tae Yeon Yoo, Reza Farhadifar, Thomas Müller-Reichert, Daniel Needleman
doi: https://doi.org/10.1101/537290
Che-Hang Yu
1John A. Paulson School of Engineering and Applied Sciences, Harvard University, USA
2Electrical and Computer Engineering, University of California Santa Barbara, USA
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  • For correspondence: chehangyu@gmail.com
Stefanie Redemann
3Experimental Center, Faculty of Medicine Carl Gustav Carus, Technische Universität Dresden, Germany
4Center for Membrane and Cell Physiology & Department of Molecular Physiology and Biological Physics, University of Virginia, USA
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Hai-Yin Wu
5Department of Physics, Harvard University, USA
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Robert Kiewisz
3Experimental Center, Faculty of Medicine Carl Gustav Carus, Technische Universität Dresden, Germany
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Tae Yeon Yoo
6Department of Molecular and Cellular Biology, Harvard University, USA
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Reza Farhadifar
1John A. Paulson School of Engineering and Applied Sciences, Harvard University, USA
6Department of Molecular and Cellular Biology, Harvard University, USA
7Center for Computational Biology, Flatiron Institute, Simons Foundation, USA
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Thomas Müller-Reichert
3Experimental Center, Faculty of Medicine Carl Gustav Carus, Technische Universität Dresden, Germany
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Daniel Needleman
1John A. Paulson School of Engineering and Applied Sciences, Harvard University, USA
6Department of Molecular and Cellular Biology, Harvard University, USA
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Abstract

Spindle microtubules, whose dynamics vary over time and at different locations, cooperatively drive chromosome segregation. Measurements of microtubule dynamics and spindle ultrastructure can provide insight into the behaviors of microtubules, helping elucidate the mechanism of chromosome segregation. Much work has focused on the dynamics and organization of kinetochore microtubules, i.e. on the region between chromosomes and poles. In comparison, microtubules in the central spindle region, between segregating chromosomes, have been less thoroughly characterized. Here, we report measurements of the movement of central spindle microtubules during chromosome segregation in human mitotic spindles, and Caenorhabditis elegans mitotic and female meiotic spindles. We found that these central spindle microtubules slide apart at the same speed as chromosomes, even as chromosomes move towards spindle poles. In these systems, damaging central spindle microtubules by laser ablation caused an immediate and complete cessation of chromosome motion, suggesting a strong coupling between central spindle microtubules and chromosomes. Electron tomographic reconstruction revealed that the analyzed anaphase spindles all contain microtubules with both ends between segregating chromosomes. Our results provide new dynamical, functional, and ultrastructural characterizations of central spindle microtubules during chromosome segregation in diverse spindles, and suggest that central spindle microtubules and chromosomes are strongly coupled in anaphase.

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  • ↵9 These authors co-advised this work

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Posted January 31, 2019.
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Central spindle microtubules are strongly coupled to chromosomes during both anaphase A and anaphase B
Che-Hang Yu, Stefanie Redemann, Hai-Yin Wu, Robert Kiewisz, Tae Yeon Yoo, Reza Farhadifar, Thomas Müller-Reichert, Daniel Needleman
bioRxiv 537290; doi: https://doi.org/10.1101/537290
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Central spindle microtubules are strongly coupled to chromosomes during both anaphase A and anaphase B
Che-Hang Yu, Stefanie Redemann, Hai-Yin Wu, Robert Kiewisz, Tae Yeon Yoo, Reza Farhadifar, Thomas Müller-Reichert, Daniel Needleman
bioRxiv 537290; doi: https://doi.org/10.1101/537290

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