Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Freeze-frame imaging of synaptic activity using SynTagMA

View ORCID ProfileAlberto Perez-Alvarez, View ORCID ProfileBrenna C. Fearey, View ORCID ProfileChristian Schulze, Ryan J. O’Toole, View ORCID ProfileBenjamien Moeyaert, View ORCID ProfileManuel A. Mohr, View ORCID ProfileIgnacio Arganda-Carreras, Wei Yang, View ORCID ProfileJ. Simon Wiegert, View ORCID ProfileEric R. Schreiter, View ORCID ProfileChristine E. Gee, View ORCID ProfileMichael B. Hoppa, View ORCID ProfileThomas G. Oertner
doi: https://doi.org/10.1101/538041
Alberto Perez-Alvarez
1Institute for Synaptic Physiology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Alberto Perez-Alvarez
Brenna C. Fearey
1Institute for Synaptic Physiology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Brenna C. Fearey
Christian Schulze
1Institute for Synaptic Physiology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Christian Schulze
Ryan J. O’Toole
2Department of Biological Sciences, Dartmouth College, Hanover, NH, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Benjamien Moeyaert
3HHMI, Janelia Farm Research Campus, VA, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Benjamien Moeyaert
Manuel A. Mohr
3HHMI, Janelia Farm Research Campus, VA, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Manuel A. Mohr
Ignacio Arganda-Carreras
4Ikerbasque, Basque Foundation for Science, Bilbao, Spain
5Dept. of Computer Science and Artificial Intelligence, Basque Country University, San Sebastian, Spain
6Donostia International Physics Center (DIPC), San Sebastian, Spain
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Ignacio Arganda-Carreras
Wei Yang
7Research Group Synaptic Wiring and Information Processing, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
J. Simon Wiegert
7Research Group Synaptic Wiring and Information Processing, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for J. Simon Wiegert
Eric R. Schreiter
3HHMI, Janelia Farm Research Campus, VA, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Eric R. Schreiter
Christine E. Gee
1Institute for Synaptic Physiology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Christine E. Gee
Michael B. Hoppa
2Department of Biological Sciences, Dartmouth College, Hanover, NH, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Michael B. Hoppa
Thomas G. Oertner
1Institute for Synaptic Physiology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Thomas G. Oertner
  • For correspondence: thomas.oertner@zmnh.uni-hamburg.de
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

ABSTRACT

Information within the brain travels from neuron to neuron across synapses. At any given moment, only a few synapses within billions will be active and are thought to transmit key information about the environment, a behavior being executed or memory being recalled. Here we present SynTagMA, which marks active synapses within a ~2 s time window. Upon violet illumination, the genetically expressed tag converts from green to red fluorescence if bound to calcium. Targeted to presynaptic terminals, preSynTagMA allows discrimination between active and silent axons. Targeted to excitatory postsynapses, postSynTagMA creates a snapshot of synapses active just before photoconversion. To analyze large datasets, we developed an analysis program that automatically identifies and tracks the fluorescence of thousands of individual synapses in tissue. Together, these tools provide a high throughput method for repeatedly mapping active synapses in vitro and in vivo.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted February 01, 2019.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Freeze-frame imaging of synaptic activity using SynTagMA
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Freeze-frame imaging of synaptic activity using SynTagMA
Alberto Perez-Alvarez, Brenna C. Fearey, Christian Schulze, Ryan J. O’Toole, Benjamien Moeyaert, Manuel A. Mohr, Ignacio Arganda-Carreras, Wei Yang, J. Simon Wiegert, Eric R. Schreiter, Christine E. Gee, Michael B. Hoppa, Thomas G. Oertner
bioRxiv 538041; doi: https://doi.org/10.1101/538041
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
Freeze-frame imaging of synaptic activity using SynTagMA
Alberto Perez-Alvarez, Brenna C. Fearey, Christian Schulze, Ryan J. O’Toole, Benjamien Moeyaert, Manuel A. Mohr, Ignacio Arganda-Carreras, Wei Yang, J. Simon Wiegert, Eric R. Schreiter, Christine E. Gee, Michael B. Hoppa, Thomas G. Oertner
bioRxiv 538041; doi: https://doi.org/10.1101/538041

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Neuroscience
Subject Areas
All Articles
  • Animal Behavior and Cognition (4683)
  • Biochemistry (10360)
  • Bioengineering (7675)
  • Bioinformatics (26337)
  • Biophysics (13528)
  • Cancer Biology (10686)
  • Cell Biology (15440)
  • Clinical Trials (138)
  • Developmental Biology (8497)
  • Ecology (12821)
  • Epidemiology (2067)
  • Evolutionary Biology (16858)
  • Genetics (11399)
  • Genomics (15478)
  • Immunology (10617)
  • Microbiology (25217)
  • Molecular Biology (10223)
  • Neuroscience (54471)
  • Paleontology (401)
  • Pathology (1668)
  • Pharmacology and Toxicology (2897)
  • Physiology (4342)
  • Plant Biology (9247)
  • Scientific Communication and Education (1586)
  • Synthetic Biology (2558)
  • Systems Biology (6781)
  • Zoology (1466)