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Freeze-frame imaging of synaptic activity using SynTagMA

View ORCID ProfileAlberto Perez-Alvarez, View ORCID ProfileBrenna Fearey, View ORCID ProfileChristian Schulze, Ryan J O'Toole, View ORCID ProfileBenjamien Moeyaert, View ORCID ProfileManuel Alexander Mohr, View ORCID ProfileIgnacio Arganda-Carreras, Wei Yang, View ORCID ProfileJ. Simon Wiegert, View ORCID ProfileEric R Schreiter, View ORCID ProfileChristine E Gee, View ORCID ProfileMichael B Hoppa, View ORCID ProfileThomas G Oertner
doi: https://doi.org/10.1101/538041
Alberto Perez-Alvarez
University Medical Center Hamburg-Eppendorf;
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Brenna Fearey
University Medical Center Hamburg-Eppendorf;
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Christian Schulze
University Medical Center Hamburg-Eppendorf;
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Ryan J O'Toole
Dartmouth College, Hanover, NH, USA;
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Benjamien Moeyaert
Howard Hughes Medical Institute;
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Manuel Alexander Mohr
Howard Hughes Medical Institute;
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Ignacio Arganda-Carreras
Basque Country University, San Sebastian, Spain
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Wei Yang
University Medical Center Hamburg-Eppendorf;
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J. Simon Wiegert
University Medical Center Hamburg-Eppendorf;
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Eric R Schreiter
Howard Hughes Medical Institute;
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Christine E Gee
University Medical Center Hamburg-Eppendorf;
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Michael B Hoppa
Dartmouth College, Hanover, NH, USA;
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Thomas G Oertner
University Medical Center Hamburg-Eppendorf;
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  • For correspondence: thomas.oertner@zmnh.uni-hamburg.de
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Abstract

Information within the brain travels from neuron to neuron across synapses. At any given moment, only a few synapses within billions will be active and are thought to transmit key information about the environment, a behavior being executed or memory being recalled. Here we present SynTagMA, which marks active synapses within a ~2 s time window. Upon violet illumination, the genetically expressed tag converts from green to red fluorescence if bound to calcium. Targeted to presynaptic terminals, preSynTagMA allows discrimination between active and silent axons. Targeted to excitatory postsynapses, postSynTagMA creates a snapshot of synapses active just before photoconversion. To analyze large datasets, we developed an analysis program that automatically identifies and tracks the fluorescence of thousands of individual synapses in tissue. Together, these tools provide a high throughput method for repeatedly mapping active synapses in vitro and in vivo.

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Posted February 01, 2019.
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Freeze-frame imaging of synaptic activity using SynTagMA
Alberto Perez-Alvarez, Brenna Fearey, Christian Schulze, Ryan J O'Toole, Benjamien Moeyaert, Manuel Alexander Mohr, Ignacio Arganda-Carreras, Wei Yang, J. Simon Wiegert, Eric R Schreiter, Christine E Gee, Michael B Hoppa, Thomas G Oertner
bioRxiv 538041; doi: https://doi.org/10.1101/538041
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Freeze-frame imaging of synaptic activity using SynTagMA
Alberto Perez-Alvarez, Brenna Fearey, Christian Schulze, Ryan J O'Toole, Benjamien Moeyaert, Manuel Alexander Mohr, Ignacio Arganda-Carreras, Wei Yang, J. Simon Wiegert, Eric R Schreiter, Christine E Gee, Michael B Hoppa, Thomas G Oertner
bioRxiv 538041; doi: https://doi.org/10.1101/538041

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