Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

A Genome-Wide Association Study of Non-Photochemical Quenching in response to local seasonal climates in Arabidopsis thaliana

Tepsuda Rungrat, Andrew A. Almonte, Riyan Cheng, Peter J. Gollan, Tim Stuart, Eva-Mari Aro, Justin O. Borevitz, Barry Pogson, Pip B. Wilson
doi: https://doi.org/10.1101/539379
Tepsuda Rungrat
1Faculty of Agriculture, Natural resources and Environment, Naresuan University, Thailand;
2ARC Centre of Excellence for Plant Energy Biology, Australian National University, Canberra, Australia;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Andrew A. Almonte
2ARC Centre of Excellence for Plant Energy Biology, Australian National University, Canberra, Australia;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Riyan Cheng
3Department of Psychiatry, University of California San Diego, La Jolla, CA 92093, USA;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Peter J. Gollan
4Faculty of Engineering and Science, University of Turku, Turku, Finland;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Tim Stuart
5ARC Centre of Excellence for Plant Energy Biology, University of Western Australia, Perth, Australia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Eva-Mari Aro
4Faculty of Engineering and Science, University of Turku, Turku, Finland;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Justin O. Borevitz
2ARC Centre of Excellence for Plant Energy Biology, Australian National University, Canberra, Australia;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Barry Pogson
2ARC Centre of Excellence for Plant Energy Biology, Australian National University, Canberra, Australia;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Pip B. Wilson
2ARC Centre of Excellence for Plant Energy Biology, Australian National University, Canberra, Australia;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: pipbwilson@gmail.com
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

Field-grown plants have variable exposure to sunlight as a result of shifting cloud-cover, seasonal changes, canopy shading, and other environmental factors. As a result, they need to have developed a method for dissipating excess energy obtained from periodic excessive sunlight exposure. Non-photochemical quenching (NPQ) dissipates excess energy as heat, however the physical and molecular genetic mechanics of NPQ variation are not understood. In this study, we investigated the genetic loci involved in NPQ by first growing different Arabidopsis thaliana accessions in local and seasonal climate conditions, then measured their NPQ kinetics through development by chlorophyll fluorescence. We used genome-wide association studies (GWAS) to identify 15 significant quantitative trait loci (QTL) for a range of photosynthetic traits, including a QTL co-located with known NPQ gene PSBS (AT1G44575). We found there were large alternative regulatory segments between the PSBS promoter regions of the functional haplotypes and a significant difference in PsbS protein concentration. These findings parallel studies in rice showing recurrent regulatory evolution of this gene. The variation in the PSBS promoter and the changes underlying other QTLs could give insight to allow manipulations of NPQ in crops to improve their photosynthetic efficiency and yield.

B.P. & J.B. conceived the project; B.P., J.B., P.W. and T.R. designed the research plan and analysis; P.W. supervised the experiments; T.R. performed most of the experiments and analysis; P.G., T.S., A.A. & E.A. designed and undertook experimental design, experiments and analysis for Figure 4; R.C. did the GWAS analysis; P.W., T.R. & A.A. wrote the article with contributions of all the authors.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Back to top
PreviousNext
Posted February 03, 2019.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
A Genome-Wide Association Study of Non-Photochemical Quenching in response to local seasonal climates in Arabidopsis thaliana
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
A Genome-Wide Association Study of Non-Photochemical Quenching in response to local seasonal climates in Arabidopsis thaliana
Tepsuda Rungrat, Andrew A. Almonte, Riyan Cheng, Peter J. Gollan, Tim Stuart, Eva-Mari Aro, Justin O. Borevitz, Barry Pogson, Pip B. Wilson
bioRxiv 539379; doi: https://doi.org/10.1101/539379
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
A Genome-Wide Association Study of Non-Photochemical Quenching in response to local seasonal climates in Arabidopsis thaliana
Tepsuda Rungrat, Andrew A. Almonte, Riyan Cheng, Peter J. Gollan, Tim Stuart, Eva-Mari Aro, Justin O. Borevitz, Barry Pogson, Pip B. Wilson
bioRxiv 539379; doi: https://doi.org/10.1101/539379

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Plant Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (4384)
  • Biochemistry (9610)
  • Bioengineering (7104)
  • Bioinformatics (24898)
  • Biophysics (12632)
  • Cancer Biology (9974)
  • Cell Biology (14373)
  • Clinical Trials (138)
  • Developmental Biology (7966)
  • Ecology (12126)
  • Epidemiology (2067)
  • Evolutionary Biology (16002)
  • Genetics (10936)
  • Genomics (14756)
  • Immunology (9880)
  • Microbiology (23699)
  • Molecular Biology (9490)
  • Neuroscience (50925)
  • Paleontology (370)
  • Pathology (1541)
  • Pharmacology and Toxicology (2687)
  • Physiology (4023)
  • Plant Biology (8674)
  • Scientific Communication and Education (1512)
  • Synthetic Biology (2402)
  • Systems Biology (6444)
  • Zoology (1346)