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Transcriptional kinetics of X-chromosome upregulation

Anton J. M. Larsson, Christos Coucoravas, Rickard Sandberg, Björn Reinius
doi: https://doi.org/10.1101/540104
Anton J. M. Larsson
1Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm Sweden.
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Christos Coucoravas
2Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm Sweden.
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Rickard Sandberg
1Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm Sweden.
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Björn Reinius
2Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm Sweden.
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  • For correspondence: bjorn.reinius@ki.se
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Abstract

Ohno’s hypothesis postulates that X-chromosome upregulation rectifies X-dose imbalance relative to autosomal genes, present in two active copies per cell. Here we dissected X-upregulation into kinetics of transcription, inferred from allele-specific single-cell RNA-sequencing (scRNAseq) data from somatic mouse cells. We confirmed increased X-chromosome expression, and remarkably found that the X-chromosome achieved upregulation by elevated burst frequencies. This provides mechanistic insights into X-chromosome upregulation.

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Posted February 05, 2019.
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Transcriptional kinetics of X-chromosome upregulation
Anton J. M. Larsson, Christos Coucoravas, Rickard Sandberg, Björn Reinius
bioRxiv 540104; doi: https://doi.org/10.1101/540104
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Transcriptional kinetics of X-chromosome upregulation
Anton J. M. Larsson, Christos Coucoravas, Rickard Sandberg, Björn Reinius
bioRxiv 540104; doi: https://doi.org/10.1101/540104

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