Abstract
Improving the end-use quality traits is one of the primary objectives in wheat breeding programs. In the current study, a population of 127 recombinant inbred lines (RILs) derived from a cross between Glenn (PI-639273) and Traverse (PI-642780) was developed and used to identify quantitative trait loci (QTL) for 16 end-use quality traits in wheat. The phenotyping of these 16 traits was performed in nine environments in North Dakota, USA. The genotyping for the RIL population was conducted using the wheat Illumina iSelect 90K SNP assay. A high-density genetic linkage map consisting of 7,963 SNP markers identified a total of 76 additive QTL (A-QTL) and 73 digenic epistatic QTL (DE-QTL) associated with these traits. Overall, 12 stable major A-QTL and three stable DE-QTL were identified for these traits, suggesting that both A-QTL and DE-QTL played an important role in controlling end-use quality traits in wheat. The most significant A-QTL (AQ.MMLPT.ndsu.1B) was detected on chromosome 1B for mixograph middle line peak time. The AQ.MMLPT.ndsu.1B A-QTL was located very close to the position of the Glu-B1 gene encoding for a subunit of high molecular weight glutenin and explained up to 24.43% of phenotypic variation for mixograph MID line peak time. A total of 23 co-localized QTL loci were detected, suggesting the possibility of the simultaneous improvement of the end-use quality traits through selection procedures in wheat breeding programs. Overall, the information provided in this study could be used in marker-assisted selection to increase selection efficiency and to improve the end-use quality in wheat.
- Abbreviations
- AACCI
- American Association of Cereal Chemists International
- A-QTL
- additive QTL
- BA
- baking absorption
- BLV
- bread loaf volume
- BLUP
- best linear unbiased predictor
- BMT
- bake-mixing time
- CBCL
- crumb color
- CTCL
- crust color
- cM
- centimorgans
- DArT
- diversity arrays technology
- DE-QTL
- digenic epistatic QTL
- DO
- dough character
- FE
- flour extraction
- FHB
- Fusarium head blight
- GPC
- grain protein content
- HMW
- high molecular weight
- HRSW
- hard red spring wheat
- ICIM-ADD
- inclusive composite interval mapping with additive effects
- ICIM-EPI
- inclusive composite interval mapping of epistatic QTL
- LMW
- low molecular weight
- MAS
- marker-assisted selection
- MELS
- mixograph envelope left slope
- MERS
- mixograph envelope right slope
- MMLPI
- mixograph MID line peak integral
- MMLPT
- mixograph MID line peak time
- MMLPV
- mixograph MID line peak value
- MMLPW
- mixograph MID line peak width
- MMLTV
- mixograph MID line time * value
- MIXOPA
- general mixograph pattern
- NDSU
- North Dakota State University
- NIR
- near-infrared reflectance
- PPM
- parts per million
- PV
- phenotypic variation
- QTL
- quantitative trait loci
- RCBD
- randomized complete block design
- RFLP
- restriction fragment length polymorphisms
- REML
- restricted maximum likelihood
- SSD
- single seed descent
- SKB
- Sandstedt, Kneen, and Blish
Footnotes
↵1 Sepehr Mohajeri Naraghi1, PhD in Plant Breeding and Genetics. Department of Plant Sciences at North Dakota State University, Fargo, ND, 58102. Email: Sepehr.mohajerinarag{at}ndsu.edu
2 Senay Simsek, PhD in Food Science. Department of Plant Sciences at North Dakota State University, Fargo, ND, 58102. Email: Senay.Simsek{at}ndsu.edu
3 Ajay Kumar, PhD in Genetics and Plant Breeding. Department of Plant Sciences at North Dakota State University, Fargo, ND, 58102. Email: ajay.kumar.2{at}ndsu.edu
4 S.M. Hisam Al Rabbi, PhD in Plant Breeding and Genetics. Department of Plant Sciences at North Dakota State University, Fargo, ND, 58102. Email: smhisam.rabbi{at}ndus.edu
5 Mohammed S. Alamri, PhD in Cereal and Food Sciences. Department of Food Sciences and Nutrition,College of Food and Agriculture Sciences, King Saud University. P. O. Box 2460 Riyadh 11451 Saudi Arabia. Email: msalamri{at}ksu.edu.sa
6 Elias. M. Elias. PhD in Plant Breeding and Genetics. Department of Plant Sciences at North Dakota State University, Fargo, ND, 58102. Email: Elias.Elias{at}ndsu.edu
7 Mohamed Mergoum, PhD in Plant Breeding and Genetics. Department of Crop and Soil Sciences, Redding Building #262, 1109 Experiment Street, Griffin, GA 30223-1797. Email: mmergoum{at}uga.edu