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Microfluidic-Induced Sleep: A Spontaneous C. elegans Sleep State Regulated by Satiety, Thermosensation and Mechanosensation

View ORCID ProfileDaniel L. Gonzales, Jasmine Zhou, Bo Fan, Jacob T. Robinson
doi: https://doi.org/10.1101/547075
Daniel L. Gonzales
1Applied Physics Program, Rice University, 6100 Main St., Houston, TX 77005, USA
2Department of Electrical and Computer Engineering, Rice University, 6100 Main St., Houston, TX 77005, USA
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  • ORCID record for Daniel L. Gonzales
Jasmine Zhou
3Department of Bioengineering, Rice University, 6100 Main St., Houston, TX 77005, USA
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Bo Fan
2Department of Electrical and Computer Engineering, Rice University, 6100 Main St., Houston, TX 77005, USA
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Jacob T. Robinson
1Applied Physics Program, Rice University, 6100 Main St., Houston, TX 77005, USA
2Department of Electrical and Computer Engineering, Rice University, 6100 Main St., Houston, TX 77005, USA
3Department of Bioengineering, Rice University, 6100 Main St., Houston, TX 77005, USA
4Department of Neuroscience, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
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  • For correspondence: jtrobinson@rice.edu
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Abstract

One remarkable feature of behavior is an animal’s ability to rapidly switch between activity states; however, how the brain regulates these spontaneous transitions based on the animal’s perceived environment is not well understood. Here we show a C. elegans sleep-like state on a scalable platform that enables simultaneous control of multiple environmental factors including temperature, mechanical stress, and food availability. This brief quiescent state, we refer to as “microfluidic-induced sleep,” occurs spontaneously in microfluidic chambers, which allows us to track animal movement and perform whole-brain imaging. With these capabilities, we establish that microfluidic-induced sleep meets the behavioral requirements of C. elegans sleep and depends on multiple factors, such as satiety and temperature. Additionally, we show for the first time that C. elegans sleep can be induced through mechanosensory pathways. Together, these results establish a rich model system for studying how animals process multiple sensory pathways to regulate behavioral states.

Footnotes

  • New analyses added to Figure 2. Several new supplemental experiments and analyses. New author added.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted August 02, 2019.
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Microfluidic-Induced Sleep: A Spontaneous C. elegans Sleep State Regulated by Satiety, Thermosensation and Mechanosensation
Daniel L. Gonzales, Jasmine Zhou, Bo Fan, Jacob T. Robinson
bioRxiv 547075; doi: https://doi.org/10.1101/547075
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Microfluidic-Induced Sleep: A Spontaneous C. elegans Sleep State Regulated by Satiety, Thermosensation and Mechanosensation
Daniel L. Gonzales, Jasmine Zhou, Bo Fan, Jacob T. Robinson
bioRxiv 547075; doi: https://doi.org/10.1101/547075

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