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Multi-immersion open-top light-sheet microscope for high-throughput imaging of cleared tissues

View ORCID ProfileAdam K. Glaser, Nicholas P. Reder, Ye Chen, View ORCID ProfileChengbo Yin, Linpeng Wei, View ORCID ProfileSoyoung Kang, Lindsey A. Barner, Weisi Xie, Erin F. McCarty, Chenyi Mao, Aaron R. Halpern, View ORCID ProfileCaleb R. Stoltzfus, Jonathan S. Daniels, Michael Y. Gerner, Philip R. Nicovich, View ORCID ProfileJoshua C. Vaughan, View ORCID ProfileLawrence D. True, Jonathan T.C. Liu
doi: https://doi.org/10.1101/548107
Adam K. Glaser
1Department of Mechanical Engineering, University of Washington, Seattle, WA USA
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  • For correspondence: akglaser@uw.edu jonliu@uw.edu
Nicholas P. Reder
2Department of Pathology, University of Washington, Seattle, WA USA
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Ye Chen
1Department of Mechanical Engineering, University of Washington, Seattle, WA USA
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Chengbo Yin
1Department of Mechanical Engineering, University of Washington, Seattle, WA USA
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Linpeng Wei
1Department of Mechanical Engineering, University of Washington, Seattle, WA USA
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Soyoung Kang
1Department of Mechanical Engineering, University of Washington, Seattle, WA USA
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Lindsey A. Barner
1Department of Mechanical Engineering, University of Washington, Seattle, WA USA
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Weisi Xie
1Department of Mechanical Engineering, University of Washington, Seattle, WA USA
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Erin F. McCarty
2Department of Pathology, University of Washington, Seattle, WA USA
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Chenyi Mao
3Department of Chemistry, University of Washington, Seattle, WA USA
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Aaron R. Halpern
3Department of Chemistry, University of Washington, Seattle, WA USA
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Caleb R. Stoltzfus
4Department of Immunology, University of Washington, Seattle, WA USA
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  • ORCID record for Caleb R. Stoltzfus
Jonathan S. Daniels
5Applied Scientific Instrumentation, Eugene, OR USA
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Michael Y. Gerner
4Department of Immunology, University of Washington, Seattle, WA USA
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Philip R. Nicovich
6Allen Institute for Brain Science, Seattle, WA USA
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Joshua C. Vaughan
3Department of Chemistry, University of Washington, Seattle, WA USA
7Department of Physiology and Biophysics, University of Washington, Seattle, WA USA
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Lawrence D. True
2Department of Pathology, University of Washington, Seattle, WA USA
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Jonathan T.C. Liu
1Department of Mechanical Engineering, University of Washington, Seattle, WA USA
2Department of Pathology, University of Washington, Seattle, WA USA
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  • For correspondence: akglaser@uw.edu jonliu@uw.edu
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Abstract

Recent advances in optical clearing and light-sheet microscopy have provided unprecedented access to structural and molecular information from intact tissues. However, current light-sheet microscopes have imposed constraints on the size, shape, number of specimens, and compatibility with various clearing protocols. Here we present a multi-immersion open-top light-sheet microscope that enables simple mounting of multiple specimens processed with a variety of protocols, which will facilitate wider adoption by preclinical researchers and clinical laboratories.

One Sentence Summary Glaser et al. describe a multi-immersion open-top light-sheet microscope that enables simple and high-throughput imaging of large numbers of preclinical and clinical specimens prepared with a variety of clearing protocols.

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Posted February 13, 2019.
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Multi-immersion open-top light-sheet microscope for high-throughput imaging of cleared tissues
Adam K. Glaser, Nicholas P. Reder, Ye Chen, Chengbo Yin, Linpeng Wei, Soyoung Kang, Lindsey A. Barner, Weisi Xie, Erin F. McCarty, Chenyi Mao, Aaron R. Halpern, Caleb R. Stoltzfus, Jonathan S. Daniels, Michael Y. Gerner, Philip R. Nicovich, Joshua C. Vaughan, Lawrence D. True, Jonathan T.C. Liu
bioRxiv 548107; doi: https://doi.org/10.1101/548107
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Multi-immersion open-top light-sheet microscope for high-throughput imaging of cleared tissues
Adam K. Glaser, Nicholas P. Reder, Ye Chen, Chengbo Yin, Linpeng Wei, Soyoung Kang, Lindsey A. Barner, Weisi Xie, Erin F. McCarty, Chenyi Mao, Aaron R. Halpern, Caleb R. Stoltzfus, Jonathan S. Daniels, Michael Y. Gerner, Philip R. Nicovich, Joshua C. Vaughan, Lawrence D. True, Jonathan T.C. Liu
bioRxiv 548107; doi: https://doi.org/10.1101/548107

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