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Dynamic interactions of lymphatic vessels at the hair follicle stem cell niche during hair regeneration

Daniel Peña-Jimenez, View ORCID ProfileSilvia Fontenete, View ORCID ProfileDiego Megias, View ORCID ProfileCoral Fustero-Torre, Osvaldo Graña-Castro, View ORCID ProfileDonatello Castellana, Robert Loewe, View ORCID ProfileMirna Perez-Moreno
doi: https://doi.org/10.1101/548768
Daniel Peña-Jimenez
1Epithelial Cell Biology Group, Cancer Cell Biology Programme, Spanish Cancer Research Centre (CNIO), Madrid, 28029, Spain.
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Silvia Fontenete
1Epithelial Cell Biology Group, Cancer Cell Biology Programme, Spanish Cancer Research Centre (CNIO), Madrid, 28029, Spain.
2Section of Cell Biology and Physiology. Department of Biology, University of Copenhagen, Copenhagen, 2100, Denmark.
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Diego Megias
3Confocal Microscopy Core Unit, Biotechnology Programme, Spanish Cancer Research Centre (CNIO), Madrid, 28029, Spain.
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Coral Fustero-Torre
4Bioinformatics Unit. Structural Biology and Biocomputing Programme, Spanish Cancer Research Centre (CNIO), Madrid, 28029, Spain.
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Osvaldo Graña-Castro
4Bioinformatics Unit. Structural Biology and Biocomputing Programme, Spanish Cancer Research Centre (CNIO), Madrid, 28029, Spain.
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Donatello Castellana
1Epithelial Cell Biology Group, Cancer Cell Biology Programme, Spanish Cancer Research Centre (CNIO), Madrid, 28029, Spain.
6Center for Cooperative Research Biosciences (CICbioGUNE). Derio Bizkaia, 48160, Spain.
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Robert Loewe
5Department of Dermatology, Medical University of Vienna, Vienna, 1090, Austria.
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Mirna Perez-Moreno
1Epithelial Cell Biology Group, Cancer Cell Biology Programme, Spanish Cancer Research Centre (CNIO), Madrid, 28029, Spain.
2Section of Cell Biology and Physiology. Department of Biology, University of Copenhagen, Copenhagen, 2100, Denmark.
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  • For correspondence: mirna.pmoreno@bio.ku.dk
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Abstract

Lymphatic vessels (LV) are essential for skin fluid homeostasis and immune cell trafficking, but whether LV are associated with hair follicle (HF) regeneration is not known. Here, by using steady and live imaging approaches in mouse skin, we show that lymphatic capillaries distribute to the anterior permanent region of individual HF and interconnect neighboring HF at the level of the HF bulge, in a hair follicle stem cell (HFSC)-dependent manner. LV further connect individual HF in triads and dynamically flow across the skin. Interestingly, at the onset of the physiological HFSC activation, or upon pharmacological or genetic induction of HF growth, LV transiently expand their caliber suggesting an increased tissue drainage capacity. Interestingly, the physiological LV caliber increase is associated with a distinct gene expression correlated to ECM and cytoskeletal reorganization. Using mouse genetics, we show that the depletion of LV blocks the pharmacological induction of HF growth. Our findings define LV as components of the HFSC niche, coordinating HF connections at tissue-level, and provide insight into their functional contribution to HF regeneration.

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Posted February 13, 2019.
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Dynamic interactions of lymphatic vessels at the hair follicle stem cell niche during hair regeneration
Daniel Peña-Jimenez, Silvia Fontenete, Diego Megias, Coral Fustero-Torre, Osvaldo Graña-Castro, Donatello Castellana, Robert Loewe, Mirna Perez-Moreno
bioRxiv 548768; doi: https://doi.org/10.1101/548768
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Dynamic interactions of lymphatic vessels at the hair follicle stem cell niche during hair regeneration
Daniel Peña-Jimenez, Silvia Fontenete, Diego Megias, Coral Fustero-Torre, Osvaldo Graña-Castro, Donatello Castellana, Robert Loewe, Mirna Perez-Moreno
bioRxiv 548768; doi: https://doi.org/10.1101/548768

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