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Neuronal network activity controls microglial process surveillance in awake mice via norepinephrine signaling

Yong Liu, Yujiao Li, Ukpong B Eyo, Tingjun Chen, Anthony Umpierre, Jia Zhu, Dale B Bosco, Hailong Dong, View ORCID ProfileLong-Jun Wu
doi: https://doi.org/10.1101/557686
Yong Liu
Mayo Clinic;
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Yujiao Li
Mayo Clinic;
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Ukpong B Eyo
Mayo Clinic;
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Tingjun Chen
Mayo Clinic;
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Anthony Umpierre
Mayo Clinic;
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Jia Zhu
Mayo Clinic;
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Dale B Bosco
Mayo Clinic;
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Hailong Dong
Fourth Military Medical University
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Long-Jun Wu
Mayo Clinic;
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  • ORCID record for Long-Jun Wu
  • For correspondence: wu.longjun@mayo.edu
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Abstract

Microglia are resident immune cells that dynamically survey the brain parenchyma, interacting with neurons in both health and disease. However, it is still unclear how neuronal network activity drives microglial dynamics. Utilizing in vivo two-photon imaging of microglia in awake mice, we found that inhibition of neuronal activity under general anesthesia dramatically increased microglial process surveillance. Accordingly, both sensory deprivation and optogenetic inhibition of local neuronal network activity in awake mice resulted in similar increases in microglial process surveillance. We further determined that reduced norepinephrine signaling is responsible for the observed increase in microglial process surveillance. Our results demonstrate that microglial process dynamics are directly influenced by neural activities through norepinephrine signaling in awake animals and indicate the importance of awake imaging for studying microglia-neuron interactions.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted February 22, 2019.
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Neuronal network activity controls microglial process surveillance in awake mice via norepinephrine signaling
Yong Liu, Yujiao Li, Ukpong B Eyo, Tingjun Chen, Anthony Umpierre, Jia Zhu, Dale B Bosco, Hailong Dong, Long-Jun Wu
bioRxiv 557686; doi: https://doi.org/10.1101/557686
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Neuronal network activity controls microglial process surveillance in awake mice via norepinephrine signaling
Yong Liu, Yujiao Li, Ukpong B Eyo, Tingjun Chen, Anthony Umpierre, Jia Zhu, Dale B Bosco, Hailong Dong, Long-Jun Wu
bioRxiv 557686; doi: https://doi.org/10.1101/557686

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