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Optogenetic olfactory behavior depends on illumination characteristics

View ORCID ProfileTayfun Tumkaya, James Stewart, Safwan B. Burhanudin, View ORCID ProfileAdam Claridge-Chang
doi: https://doi.org/10.1101/559674
Tayfun Tumkaya
1Institute for Molecular and Cell Biology, A*STAR, Singapore 138673
2Department of Physiology, National University of Singapore, Singapore
3Program in Neuroscience and Behavioral Disorders, Duke-NUS Medical School, Singapore
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James Stewart
1Institute for Molecular and Cell Biology, A*STAR, Singapore 138673
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Safwan B. Burhanudin
1Institute for Molecular and Cell Biology, A*STAR, Singapore 138673
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Adam Claridge-Chang
1Institute for Molecular and Cell Biology, A*STAR, Singapore 138673
2Department of Physiology, National University of Singapore, Singapore
3Program in Neuroscience and Behavioral Disorders, Duke-NUS Medical School, Singapore
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Abstract

Optogenetics has become an important tool for the study of behavior, enabling neuroscientists to infer causations by examining behavior after activating genetically circumscribed neurons with light. Light-induced neural activity is affected by illumination parameters used in experiments, such as intensity, duration, and frequency. Here, we hypothesized that the intensity of light and the presence of oscillations in illumination would alter optogenetically induced olfactory behaviours. To test this, we activated olfactory receptor neurons (ORNs) in Drosophila by using either static or pulsed light stimuli across a range of light intensities. The various regimes elicited distinct behavioral valence responses (attraction, aversion, indifference) from several ORN types. Our results demonstrate the importance of both frequency and intensity for interpreting optogenetic behavioral experiments accurately; successfully generalizing optogenetic results requires the use of more than a single illumination regime.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted February 24, 2019.
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Optogenetic olfactory behavior depends on illumination characteristics
Tayfun Tumkaya, James Stewart, Safwan B. Burhanudin, Adam Claridge-Chang
bioRxiv 559674; doi: https://doi.org/10.1101/559674
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Optogenetic olfactory behavior depends on illumination characteristics
Tayfun Tumkaya, James Stewart, Safwan B. Burhanudin, Adam Claridge-Chang
bioRxiv 559674; doi: https://doi.org/10.1101/559674

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