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A high-density genetic map and molecular sex-typing assay for gerbils

View ORCID ProfileThomas D. Brekke, Sushmita Supriya, Megan Denver, Angharad Thom, View ORCID ProfileKatherine A. Steele, View ORCID ProfileJohn F. Mulley
doi: https://doi.org/10.1101/567016
Thomas D. Brekke
1School of Natural Sciences, Bangor University, Bangor, Gwynedd, LL57 2DG, United Kingdom
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Sushmita Supriya
1School of Natural Sciences, Bangor University, Bangor, Gwynedd, LL57 2DG, United Kingdom
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Megan Denver
1School of Natural Sciences, Bangor University, Bangor, Gwynedd, LL57 2DG, United Kingdom
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Angharad Thom
1School of Natural Sciences, Bangor University, Bangor, Gwynedd, LL57 2DG, United Kingdom
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Katherine A. Steele
1School of Natural Sciences, Bangor University, Bangor, Gwynedd, LL57 2DG, United Kingdom
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John F. Mulley
1School of Natural Sciences, Bangor University, Bangor, Gwynedd, LL57 2DG, United Kingdom
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  • For correspondence: j.mulley@bangor.ac.uk
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Abstract

We constructed a high-density genetic map in Mongolian gerbils (Meriones unguiculatus). We genotyped 137 F2 individuals with a genotype-by-sequencing (GBS) approach at over 10,000 loci and built the genetic map using a two-step approach. First, we chose the highest-quality set of 485 markers to construct a high-quality framework map of 1,239cM with 22 linkage groups as expected from the published karyotype. Second, we added an additional 5,449 markers onto the map based on their genotype similarity with the original markers. We used the final marker set to assemble 1,140 genomic scaffolds (containing ∼20% of annotated genes) into a chromosome-level framework. We used both genetic linkage and relative sequencing coverage in males and females to identify X- and Y-chromosome scaffolds and from these we designed a robust and internally-controlled PCR assay to determine sex. This assay will facilitate early stage sex-typing of embryonic and young gerbils which is not possible using current visual methods.

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Posted March 05, 2019.
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A high-density genetic map and molecular sex-typing assay for gerbils
Thomas D. Brekke, Sushmita Supriya, Megan Denver, Angharad Thom, Katherine A. Steele, John F. Mulley
bioRxiv 567016; doi: https://doi.org/10.1101/567016
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A high-density genetic map and molecular sex-typing assay for gerbils
Thomas D. Brekke, Sushmita Supriya, Megan Denver, Angharad Thom, Katherine A. Steele, John F. Mulley
bioRxiv 567016; doi: https://doi.org/10.1101/567016

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