Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Early changes in the urine proteome in a rat liver tumor model

Yameng Zhang, Yufei Gao, Youhe Gao
doi: https://doi.org/10.1101/568246
Yameng Zhang
Department of Biochemistry and Molecular Biology, College of Life Sciences, Beijing Normal University, Gene Engineering Drug and Biotechnology Beijing Key Laboratory, Beijing 100875, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yufei Gao
College of Information Science and Technology, Beijing Normal University, Beijing 100875, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Youhe Gao
Department of Biochemistry and Molecular Biology, College of Life Sciences, Beijing Normal University, Gene Engineering Drug and Biotechnology Beijing Key Laboratory, Beijing 100875, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: gaoyouhe@bnu.edu.cn
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

Urine, as a potential biomarker source among the body fluids, can accumulate many changes in the body due to the lack of a mechanism to maintain a homeostatic state. Previous studies have demonstrated that proteomic technology can find many potential biomarkers to reflect different diseases in the urine. This study aims to detect early changes in the urinary proteome in a rat liver tumor model. The tumor model was established with the Walker-256 carcinosarcoma cell line (W256). Compared to before the injection, ninety-five differential proteins were significantly changed in the experimental rats. At day 3, twelve proteins were identified in the absence of pathological changes, and four of them were altered at all four time-points (B2MG, VCAM1, HA11, and LG3BP). Seven had previously been associated with liver cancer. At day 5, fifty-two differential proteins were identified. At day 7 and day 11, there was a significant decrease in the body weight of the rats, and tumor tissue was observed in the liver. Fifty-two and forty differential proteins were changed significantly at day 7 and day 11, respectively. Of the proteins that were identified at these three time-points, and twenty-four were reported to be associated with liver cancer. Comparing the differential urinary proteins and biological processes of liver tumor model with those in different models of W256 grown in other organs, specific differential protein patterns were found among the four models, which indicates that the differential urinary proteins can reflect the differences when the same tumor cell grown in different organs.

Significance This study demonstrated that (1) the rat liver tumor model caused early changes in urinary proteins may give new insight into the early diagnosis of liver cancer; (2) the same tumor cell grown in different organs can be reflected in differential urinary proteins.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted March 05, 2019.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Early changes in the urine proteome in a rat liver tumor model
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
Share
Early changes in the urine proteome in a rat liver tumor model
Yameng Zhang, Yufei Gao, Youhe Gao
bioRxiv 568246; doi: https://doi.org/10.1101/568246
Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
Early changes in the urine proteome in a rat liver tumor model
Yameng Zhang, Yufei Gao, Youhe Gao
bioRxiv 568246; doi: https://doi.org/10.1101/568246

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Biochemistry
Subject Areas
All Articles
  • Animal Behavior and Cognition (1546)
  • Biochemistry (2502)
  • Bioengineering (1759)
  • Bioinformatics (9734)
  • Biophysics (3930)
  • Cancer Biology (2996)
  • Cell Biology (4237)
  • Clinical Trials (135)
  • Developmental Biology (2655)
  • Ecology (4133)
  • Epidemiology (2033)
  • Evolutionary Biology (6936)
  • Genetics (5247)
  • Genomics (6534)
  • Immunology (2209)
  • Microbiology (7019)
  • Molecular Biology (2786)
  • Neuroscience (17431)
  • Paleontology (127)
  • Pathology (433)
  • Pharmacology and Toxicology (712)
  • Physiology (1069)
  • Plant Biology (2516)
  • Scientific Communication and Education (647)
  • Synthetic Biology (836)
  • Systems Biology (2700)
  • Zoology (439)