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Transcriptome-wide dynamics of extensive m6A mRNA methylation during Plasmodium falciparum blood-stage development

View ORCID ProfileSebastian Baumgarten, Jessica M. Bryant, Ameya Sinha, Thibaud Reyser, Peter R. Preiser, Peter C. Dedon, Artur Scherf
doi: https://doi.org/10.1101/572891
Sebastian Baumgarten
1Biology of Host-Parasite Interactions Unit, Department of Parasites and Insect Vectors, Institut Pasteur, 75015 Paris, France
2CNRS, ERL 9195, 75015 Paris, France
3INSERM, Unit U1201, 75015 Paris, France
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  • ORCID record for Sebastian Baumgarten
  • For correspondence: artur.scherf@pasteur.fr sebastian.baumgarten@pasteur.fr
Jessica M. Bryant
1Biology of Host-Parasite Interactions Unit, Department of Parasites and Insect Vectors, Institut Pasteur, 75015 Paris, France
2CNRS, ERL 9195, 75015 Paris, France
3INSERM, Unit U1201, 75015 Paris, France
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Ameya Sinha
4School of Biological Sciences, Nanyang Technological University, Singapore 637551, Singapore
5Antimicrobial Resistance Interdisciplinary Research Group, Singapore-MIT Alliance for Research and Technology, Singapore 138602, Singapore
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Thibaud Reyser
1Biology of Host-Parasite Interactions Unit, Department of Parasites and Insect Vectors, Institut Pasteur, 75015 Paris, France
2CNRS, ERL 9195, 75015 Paris, France
3INSERM, Unit U1201, 75015 Paris, France
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Peter R. Preiser
4School of Biological Sciences, Nanyang Technological University, Singapore 637551, Singapore
5Antimicrobial Resistance Interdisciplinary Research Group, Singapore-MIT Alliance for Research and Technology, Singapore 138602, Singapore
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Peter C. Dedon
5Antimicrobial Resistance Interdisciplinary Research Group, Singapore-MIT Alliance for Research and Technology, Singapore 138602, Singapore
6Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA
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Artur Scherf
1Biology of Host-Parasite Interactions Unit, Department of Parasites and Insect Vectors, Institut Pasteur, 75015 Paris, France
2CNRS, ERL 9195, 75015 Paris, France
3INSERM, Unit U1201, 75015 Paris, France
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  • For correspondence: artur.scherf@pasteur.fr sebastian.baumgarten@pasteur.fr
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Abstract

Malaria pathogenesis results from the asexual replication of Plasmodium falciparum within human red blood cells, which relies on a precisely timed cascade of gene expression over a 48-hour life cycle. Although substantial post-transcriptional regulation of this hardwired program has been observed, it remains unclear how these processes are mediated on a transcriptome-wide level. To this end, we identified mRNA modifications in the P. falciparum transcriptome and performed a comprehensive characterization of N6-methyladenosine (m6A) over the course of blood stage development. Using mass spectrometry and m6A RNA sequencing, we demonstrate that m6A is highly developmentally regulated, exceeding m6A levels known in any other eukaryote. We identify an evolutionarily conserved m6A writer complex and show that knockdown of the putative m6A methyltransferase by CRISPR interference leads to increased levels of transcripts that normally contain m6A. In accordance, we find an inverse correlation between m6A status and mRNA stability or translational efficiency. Our data reveal the crucial role of extensive m6A mRNA methylation in dynamically fine-tuning the transcriptional program of a unicellular eukaryote as well as a new ‘epitranscriptomic’ layer of gene regulation in malaria parasites.

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Posted March 09, 2019.
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Transcriptome-wide dynamics of extensive m6A mRNA methylation during Plasmodium falciparum blood-stage development
Sebastian Baumgarten, Jessica M. Bryant, Ameya Sinha, Thibaud Reyser, Peter R. Preiser, Peter C. Dedon, Artur Scherf
bioRxiv 572891; doi: https://doi.org/10.1101/572891
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Transcriptome-wide dynamics of extensive m6A mRNA methylation during Plasmodium falciparum blood-stage development
Sebastian Baumgarten, Jessica M. Bryant, Ameya Sinha, Thibaud Reyser, Peter R. Preiser, Peter C. Dedon, Artur Scherf
bioRxiv 572891; doi: https://doi.org/10.1101/572891

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