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Inducible overexpression of zebrafish microRNA-722 suppresses chemotaxis of human neutrophil like cells

View ORCID ProfileAlan Y. Hsu, Sheng Liu, Kent A. Brasseale, Ramizah Syahirah, Jun Wan, View ORCID ProfileQing Deng
doi: https://doi.org/10.1101/578583
Alan Y. Hsu
1Department of Biological Sciences, Purdue University, West Lafayette, IN 47907 USA
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Sheng Liu
2Department of Medical and Molecular Genetics, Indiana University School of Medicine, Indianapolis, IN 46202 USA
3Collaborative Core for Cancer Bioinformatics, Indiana University Simon Cancer Center, Indianapolis, IN 46202 USA
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Kent A. Brasseale
1Department of Biological Sciences, Purdue University, West Lafayette, IN 47907 USA
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Ramizah Syahirah
1Department of Biological Sciences, Purdue University, West Lafayette, IN 47907 USA
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Jun Wan
2Department of Medical and Molecular Genetics, Indiana University School of Medicine, Indianapolis, IN 46202 USA
3Collaborative Core for Cancer Bioinformatics, Indiana University Simon Cancer Center, Indianapolis, IN 46202 USA
4Center for Computational Biology and Bioinformatics, Indiana University School of Medicine, Indianapolis, IN 46202 USA
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Qing Deng
1Department of Biological Sciences, Purdue University, West Lafayette, IN 47907 USA
5Purdue Institute for Inflammation, Immunology, & Infectious Disease, Purdue University, West Lafayette, IN 47907 USA
6Purdue University Center for Cancer Research, Purdue University, West Lafayette, IN 47907 USA
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  • For correspondence: qingdeng@purdue.edu
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Abstract

Neutrophil migration is essential for battling against infections but also drives chronic inflammation. Since primary neutrophils are terminally differentiated and not genetically tractable, leukemia cells such as HL-60 are differentiated into neutrophil-like cells to study mechanisms underlying neutrophil migration. However, constitutive overexpression or inhibition in this cell line does not allow the characterization of the genes that affect the differentiation process. Here we apply the tet-on system to induce the expression of zebrafish miR-722 in differentiated HL-60. Overexpression of miR-722 reduced the mRNA level of genes in the chemotaxis and inflammation pathways, including RAC2. Consistently, cell migration is significantly inhibited upon induced miR-722 overexpression. Together, miR-722 is an evolutionarily conserved suppressor for neutrophil migration and signaling in zebrafish and human.

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Posted March 14, 2019.
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Inducible overexpression of zebrafish microRNA-722 suppresses chemotaxis of human neutrophil like cells
Alan Y. Hsu, Sheng Liu, Kent A. Brasseale, Ramizah Syahirah, Jun Wan, Qing Deng
bioRxiv 578583; doi: https://doi.org/10.1101/578583
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Inducible overexpression of zebrafish microRNA-722 suppresses chemotaxis of human neutrophil like cells
Alan Y. Hsu, Sheng Liu, Kent A. Brasseale, Ramizah Syahirah, Jun Wan, Qing Deng
bioRxiv 578583; doi: https://doi.org/10.1101/578583

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