Dynamic metabolic profiles of marine macroalga Ulva prolifera during fragmentation-induced proliferation

Abstract

Ulva prolifera, a type of marine macroalgae, is the causative species behind green tides mainly in the Yellow Sea and adjacent regions, nevertheless, it can be used as food or animal feed in South China. The vegetative fragments of U. prolifera are an important seed source for successive green tide blooms. Fragmentation shortens the transition time from the vegetative state to the reproductive state. However, the translation of the algal metabolites during gametogenesis was far from well understood. In this study, the dynamic metabolic profiles of U. prolifera thallus during fragmentation-induced proliferation were investigated using non-targeted metabolomics approach in a time series of experiments in June 2017. After a 30 min low temperature shock, fragmentation induced a reproductive response of 91.57% of U. prolifera in 48 h, whereas that was only 21.43% in the control group. A total of 156 chromatographic peaks were detected, and 63 metabolites were significantly changed in U. prolifera during reproduction. The results of the Kinetic metabolic pattern showed that the fragments not only induced the formation of sporangium, but also complicated their metabolites accumulation. During fragmentation-induced proliferation, U. prolifera consumed different sugars at different time points. γ-aminobutyric acid (GABA), glutamic acid, gallic acid, and malic acid may play important roles in germ cell formation and release of U. prolifera, whereas n-hexanol, 2-methyl-3-phenylindole, and 3-indoleacetonitrile may be beneficial in biotic stress resistance. Compared with the control group, the metabolites, such as alcohol and organic acid, also showed significant difference with the photoperiod at the initial stage of proliferation (before 60 h). In conclusion, that the metabolites including sugars, organic acids, and alcohol changed with different photoperiod may be the strategy for U. prolifera to cope with adverse environment and rapid proliferation.