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Direct interaction of PIWI and DEPS-1 is essential for piRNA function and condensate ultrastructure in Caenorhabditis elegans

View ORCID ProfileKM Suen, View ORCID ProfileF Braukmann, R Butler, D Bensaddek, View ORCID ProfileA Akay, C. -C Lin, N Doshi, A Sapetschnig, A Lamond, JE Ladbury, EA Miska
doi: https://doi.org/10.1101/580043
KM Suen
Wellcome Trust Cancer Research UK Gurdon Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UKDepartment of Genetics, University of Cambridge, Downing Street, Cambridge, CB2 3EH, UK
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F Braukmann
Wellcome Trust Cancer Research UK Gurdon Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UKDepartment of Genetics, University of Cambridge, Downing Street, Cambridge, CB2 3EH, UK
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R Butler
Wellcome Trust Cancer Research UK Gurdon Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK
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D Bensaddek
Laboratory for Quantitative Proteomics, Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UKBioscience Core labs, King Abdullah University of Science and Technology, Thuwal 23955-6900 Saudi Arabia
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A Akay
Wellcome Trust Cancer Research UK Gurdon Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UKDepartment of Genetics, University of Cambridge, Downing Street, Cambridge, CB2 3EH, UK
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C. -C Lin
School of Molecular and Cellular Biology, University of Leeds, LC Miall Building, Leeds, LS2 9JT, UK
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N Doshi
University of Cambridge, School of Clinical Medicine, Cambridge Biomedical Campus, Cambridge, CB2 0SP, UK
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A Sapetschnig
Wellcome Sanger Institute, Wellcome Trust Genome Campus, Hinxton, CB10 1SA, UK
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A Lamond
Laboratory for Quantitative Proteomics, Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK
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JE Ladbury
School of Molecular and Cellular Biology, University of Leeds, LC Miall Building, Leeds, LS2 9JT, UK
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EA Miska
Wellcome Trust Cancer Research UK Gurdon Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UKDepartment of Genetics, University of Cambridge, Downing Street, Cambridge, CB2 3EH, UKWellcome Sanger Institute, Wellcome Trust Genome Campus, Hinxton, CB10 1SA, UK
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Article Information

doi 
https://doi.org/10.1101/580043
History 
  • March 16, 2019.
Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.

Author Information

  1. KM Suen*1,2,
  2. F Braukmann*1,2,
  3. R Butler1,
  4. D Bensaddek4,7,
  5. A Akay1,2,
  6. C. -C Lin5,
  7. N Doshi6,
  8. A Sapetschnig3,
  9. A Lamond4,
  10. JE Ladbury5 and
  11. EA Miska1,2,3
  1. 1Wellcome Trust Cancer Research UK Gurdon Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK
  2. 2Department of Genetics, University of Cambridge, Downing Street, Cambridge, CB2 3EH, UK
  3. 3Wellcome Sanger Institute, Wellcome Trust Genome Campus, Hinxton, CB10 1SA, UK
  4. 4Laboratory for Quantitative Proteomics, Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK
  5. 5School of Molecular and Cellular Biology, University of Leeds, LC Miall Building, Leeds, LS2 9JT, UK
  6. 6University of Cambridge, School of Clinical Medicine, Cambridge Biomedical Campus, Cambridge, CB2 0SP, UK
  7. 7Bioscience Core labs, King Abdullah University of Science and Technology, Thuwal 23955-6900 Saudi Arabia
  1. Corresponding author: eam29{at}cam.ac.uk
  1. ↵* Equal contributions

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Posted March 16, 2019.
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Direct interaction of PIWI and DEPS-1 is essential for piRNA function and condensate ultrastructure in Caenorhabditis elegans
KM Suen, F Braukmann, R Butler, D Bensaddek, A Akay, C. -C Lin, N Doshi, A Sapetschnig, A Lamond, JE Ladbury, EA Miska
bioRxiv 580043; doi: https://doi.org/10.1101/580043
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Direct interaction of PIWI and DEPS-1 is essential for piRNA function and condensate ultrastructure in Caenorhabditis elegans
KM Suen, F Braukmann, R Butler, D Bensaddek, A Akay, C. -C Lin, N Doshi, A Sapetschnig, A Lamond, JE Ladbury, EA Miska
bioRxiv 580043; doi: https://doi.org/10.1101/580043

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