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Visualization of Loki- and Heimdallarchaeia (Asgardarchaeota) by fluorescence in situ hybridization and catalyzed reporter deposition (CARD-FISH)

View ORCID ProfileMichaela M. Salcher, Adrian-Ştefan Andrei, Paul-Adrian Bulzu, Zsolt G. Keresztes, Horia L. Banciu, Rohit Ghai
doi: https://doi.org/10.1101/580431
Michaela M. Salcher
1Department of Aquatic Microbial Ecology, Institute of Hydrobiology, Biology Centre of the Czech Academy of Sciences, České Budějovice, Czech Republic.
2Limnological Station, Institute of Plant and Microbial Biology, University of Zurich, Kilchberg, Switzerland.
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  • For correspondence: michaelasalcher@gmail.com
Adrian-Ştefan Andrei
1Department of Aquatic Microbial Ecology, Institute of Hydrobiology, Biology Centre of the Czech Academy of Sciences, České Budějovice, Czech Republic.
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Paul-Adrian Bulzu
3Department of Molecular Biology and Biotechnology, Faculty of Biology and Geology, Babeş-Bolyai University, Cluj-Napoca, Romania
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Zsolt G. Keresztes
3Department of Molecular Biology and Biotechnology, Faculty of Biology and Geology, Babeş-Bolyai University, Cluj-Napoca, Romania
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Horia L. Banciu
3Department of Molecular Biology and Biotechnology, Faculty of Biology and Geology, Babeş-Bolyai University, Cluj-Napoca, Romania
4Molecular Biology Center, Institute for Interdisciplinary Research in Bio-Nano-Sciences, Babeş-Bolyai University, Cluj-Napoca, Romania
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Rohit Ghai
1Department of Aquatic Microbial Ecology, Institute of Hydrobiology, Biology Centre of the Czech Academy of Sciences, České Budějovice, Czech Republic.
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Abstract

Metagenome-assembled genomes (MAGs) of Asgardarchaeota are starting to be recovered from a variety of habitats, broadening their environmental distribution and providing access to the genetic makeup of this archaeal lineage. Despite their singular phylogenetic position at the base of the eukaryotic tree of life, the morphology of these bewildering organisms remains a mystery. In order to visualize this elusive group, we applied a combination of CARD-FISH and epifluorescence microscopy on coastal hypersaline sediment samples, using specifically designed CARD-FISH probes for Heimdallarchaeia and Lokiarchaeia lineages and provide the first visual evidence for both these groups. Here, we show that while Heimdallarchaeia are characterized by a uniform cellular morphology typified by central DNA localization, Lokiarchaeia display a plethora of shapes and sizes that likely reflect their broad phylogenetic diversity and ecological distribution.

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  • Competing Interests

    The authors declare no competing financial interests in relation to the work described.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted March 17, 2019.
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Visualization of Loki- and Heimdallarchaeia (Asgardarchaeota) by fluorescence in situ hybridization and catalyzed reporter deposition (CARD-FISH)
Michaela M. Salcher, Adrian-Ştefan Andrei, Paul-Adrian Bulzu, Zsolt G. Keresztes, Horia L. Banciu, Rohit Ghai
bioRxiv 580431; doi: https://doi.org/10.1101/580431
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Visualization of Loki- and Heimdallarchaeia (Asgardarchaeota) by fluorescence in situ hybridization and catalyzed reporter deposition (CARD-FISH)
Michaela M. Salcher, Adrian-Ştefan Andrei, Paul-Adrian Bulzu, Zsolt G. Keresztes, Horia L. Banciu, Rohit Ghai
bioRxiv 580431; doi: https://doi.org/10.1101/580431

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