Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

High-throughput RNA sequencing from paired lesional- and non-lesional skin reveals major alterations in the psoriasis circRNAome

Liviu-Ionut Moldovan, View ORCID ProfileThomas Birkballe Hansen, Morten Trillingsgaard Venø, Trine Line Hauge Okholm, Thomas Levin Andersen, Henrik Hager, View ORCID ProfileLars Iversen, Jørgen Kjems, Claus Johansen, View ORCID ProfileLasse Sommer Kristensen
doi: https://doi.org/10.1101/581066
Liviu-Ionut Moldovan
Department of Molecular Biology and Genetics (MBG), Aarhus University, DK-8000, Aarhus, DenmarkInterdisciplinary Nanoscience Center (iNANO), Aarhus University, DK-8000, Aarhus, Denmark
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Thomas Birkballe Hansen
Department of Molecular Biology and Genetics (MBG), Aarhus University, DK-8000, Aarhus, DenmarkInterdisciplinary Nanoscience Center (iNANO), Aarhus University, DK-8000, Aarhus, Denmark
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Thomas Birkballe Hansen
Morten Trillingsgaard Venø
Department of Molecular Biology and Genetics (MBG), Aarhus University, DK-8000, Aarhus, DenmarkInterdisciplinary Nanoscience Center (iNANO), Aarhus University, DK-8000, Aarhus, Denmark
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Trine Line Hauge Okholm
Department of Molecular Medicine (MOMA), Aarhus University Hospital, DK-8200, Aarhus, Denmark
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Thomas Levin Andersen
Clinical Cell Biology, Department of Pathology, Vejle Hospital and Odense University Hospital, DK-5000, Odense, Denmark
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Henrik Hager
Department of Clinical Research and Department of Molecular Medicine, University of Southern Denmark, DK-5000, Odense, Denmark
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Lars Iversen
Department of Dermatology, Aarhus University Hospital, Aarhus, Denmark
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Lars Iversen
Jørgen Kjems
Department of Molecular Biology and Genetics (MBG), Aarhus University, DK-8000, Aarhus, DenmarkInterdisciplinary Nanoscience Center (iNANO), Aarhus University, DK-8000, Aarhus, Denmark
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Claus Johansen
Department of Dermatology, Aarhus University Hospital, Aarhus, Denmark
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Lasse Sommer Kristensen
Department of Molecular Biology and Genetics (MBG), Aarhus University, DK-8000, Aarhus, DenmarkInterdisciplinary Nanoscience Center (iNANO), Aarhus University, DK-8000, Aarhus, Denmark
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Lasse Sommer Kristensen
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

Background Psoriasis is a chronic inflammatory skin disease characterized by hyperproliferation and abnormal differentiation of keratinocytes. It is one of the most prevalent chronic inflammatory skin condition in adults worldwide, with a considerable negative impact on quality of life. Circular RNAs (circRNAs) are a recently identified type of non-coding RNA with diverse cellular functions related to their exceptional stability. In particular, some circRNAs can bind and regulate microRNAs (miRNAs), a group of RNAs that play a role in the pathogenesis of psoriasis. The aim of this study was to characterize the circRNAome in psoriasis and to assess potential correlations to miRNA expression patterns.

Results Using high-throughput RNA-sequencing (RNA-seq) and NanoString nCounter technology, we found a substantial down-regulation of circRNA expression in lesional skin compared to non-lesional skin from psoriasis patients. We saw that this mainly applies to the epidermis by analyzing laser capture microdissected tissues and by RNA chromogenic in situ hybridization (CISH). We also found that the majority of the circRNAs were downregulated independent of their corresponding linear host genes. The observed downregulation of circRNAs in psoriasis was not due to altered expression levels of factors known to affect circRNA biogenesis, nor because lesional skin contained an increased number of inflammatory cells such as lymphocytes. Finally, we saw that the overall differences in available miRNA binding sites on the circRNAs between lesional and non-lesional skin did not correlate with differences in miRNA expression patterns.

Conclusions We have performed the first genome-wide circRNA profiling of paired lesional and non-lesional skin from psoriasis patients and revealed that circRNAs are much less abundant in the lesional samples. Whether this is a cause or a consequence of the disease remains to be revealed, however, we found no evidence that the loss of miRNA binding sites on the circRNAs could explain differences in miRNA expression reported between lesional and non-lesional skin.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted March 21, 2019.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
High-throughput RNA sequencing from paired lesional- and non-lesional skin reveals major alterations in the psoriasis circRNAome
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
Share
High-throughput RNA sequencing from paired lesional- and non-lesional skin reveals major alterations in the psoriasis circRNAome
Liviu-Ionut Moldovan, Thomas Birkballe Hansen, Morten Trillingsgaard Venø, Trine Line Hauge Okholm, Thomas Levin Andersen, Henrik Hager, Lars Iversen, Jørgen Kjems, Claus Johansen, Lasse Sommer Kristensen
bioRxiv 581066; doi: https://doi.org/10.1101/581066
Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
High-throughput RNA sequencing from paired lesional- and non-lesional skin reveals major alterations in the psoriasis circRNAome
Liviu-Ionut Moldovan, Thomas Birkballe Hansen, Morten Trillingsgaard Venø, Trine Line Hauge Okholm, Thomas Levin Andersen, Henrik Hager, Lars Iversen, Jørgen Kjems, Claus Johansen, Lasse Sommer Kristensen
bioRxiv 581066; doi: https://doi.org/10.1101/581066

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Molecular Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (1532)
  • Biochemistry (2491)
  • Bioengineering (1747)
  • Bioinformatics (9700)
  • Biophysics (3915)
  • Cancer Biology (2978)
  • Cell Biology (4213)
  • Clinical Trials (135)
  • Developmental Biology (2639)
  • Ecology (4108)
  • Epidemiology (2033)
  • Evolutionary Biology (6910)
  • Genetics (5224)
  • Genomics (6519)
  • Immunology (2193)
  • Microbiology (6971)
  • Molecular Biology (2765)
  • Neuroscience (17348)
  • Paleontology (126)
  • Pathology (430)
  • Pharmacology and Toxicology (708)
  • Physiology (1062)
  • Plant Biology (2498)
  • Scientific Communication and Education (646)
  • Synthetic Biology (832)
  • Systems Biology (2691)
  • Zoology (432)